Total Collaborative Study Protocol_Solus One Salmonella v1 1

( k )  After heat treatment, cool the samples to room temperature (15–25°C). This may be 

accelerated by placing the test tubes in cold water for 5 min. 

( l )  Where appropriate use frit filters with denatured matrices that contain high concentrations of 

particulates to minimize sample handling errors. 

( m ) Keep the remaining non‐heat treated samples for verification until ELISA results are obtained. 

These samples can be stored at 41.5 ± 1°C if the ELISA test is to be carried out within 2 h or at 2–8°C for 

up to 72 h if not.  

Solus One Salmonella (Manual Method) 

( a ) Remove the kit from storage at 2–8°C an hour before use to allow the components to reach 

room temperature (15–25°C). Determine the number of wells required for the test. Take the required 

number of strips from the pouch and fit them to the framer provided. Note: Unused strips should be 

returned to the pouch and stored at 2–8°C. 

( b ) Prepare the Washing Buffer by adding the contents of a single Washing Buffer Activator 

sachet and Washing Buffer bottle (60 mL) to 1440 mL or a single Washing Buffer Activator sachet and 

Washing Buffer bottle (10 mL) to 240 mL of deionized water to prepare the washing buffer at assay 

concentration. Mix until the activator has fully dissolved. 

( c )  Leave the first well in the strip empty to serve as a “blank” for measuring the absorbance of  the substrate.  ( d ) Aliquot 0.1 mL of the Negative Control in the second well and 0.1 mL of the Positive Control in  the third well.  ( e ) Add 0.1 mL of each heated sample to each consecutive well in the strip. If there are wells left

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