Total Collaborative Study Protocol_Solus One Salmonella v1 1

and select the “Report” button. 

2) The report can be viewed and printed from the report display screen. 

3) The results are displayed in a 96 well format with each individual optical density reading. 

4) A secondary file which can be used to export the results into a LIMS system is created in a 

folder on the desk top called “ELISA Export Results”, this is in a CSV (comma separated 

values) file format. 

Immunoassay result interpretation 

The presence or absence of the pathogen is expressed as optical density (OD 450

) measurements 

using the Dynex DS2 instrument plate reader or a microplate reader with a 450 nm filter where a Dynex 

< 0.100 is valid 

DS2 instrument is unavailable. For control assay acceptance criteria, a result of an OD 450

for a Negative Control, whereas a result of an OD 450

> 0.500 is valid for a Positive Control. A result of an 

OD 450 < 0.200 is considered to be negative for  Salmonella . A result of an OD 450

≥ 0.200 is considered 

presumptive positive for  Salmonella . 

Confirmation 

All samples are confirmed according to the appropriate reference methods specified for the matrix 

and analyte. 

Validation Study

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