Total Collaborative Study Protocol_Solus One Salmonella v1 1
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click next.
6) Click on the Solus One Salmonella assay. Then click the “Add Assay” button.
7) Select “Done” when assay selection is completed. 8) Select the “Accept” button to start the run.
9) After the timeline has been accepted, loading of sample tube racks, reagents and
consumables is initiated.
( c )
Loading the Dynex DS2 Instrument
1) If not already onboard, load sample test tube racks into the slots. 2) Remove the lid from the pipette boxes and load into the instrument.
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3) Load the Conjugate, TMB Substrate, and Stop Solution into the correct locations specified
by the instrument.
4) Load the Negative and Positive Controls in each position specified by the instrument. 5) Prepare and dispense the correct amount of Activated Washing Buffer.
6) Verify all disposable containers do not need emptying.
( d ) Remove Samples
1) When the run is completed, open the lid to the Dynex DS2 instrument and remove the loading trays, with the sample test tubes, by pulling the loading trays out away from the instrument. Discard sample test tubes in the proper location according to laboratory
procedures.
2) Discard the reagent tubes, negative controls, and positive controls in the proper location according to laboratory procedures. Note: Reagents may be capped and refrigerated at 2–
8°C with the rest of the remaining kit.
J. Test Result Report
( a ) To view a report on a completed run, select the desired result file in the “Recent Test” Box,
and select the “Report” button.
( b ) The report can be viewed and printed from the report display screen.
( c ) The results are displayed in a 96 well format with each individual optical density reading. ( d ) A secondary file which can be used to export the results into a LIMS system is created in a 30 folder on the desk top called “ELISA Export Results”, this is in a CSV (comma separated values) file 31 format.
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K. Interpretation
The presence or absence of the pathogen is expressed as optical density (OD 450 36 using the Dynex DS2 instrument plate reader or a microplate reader with a 450 nm filter where a Dynex 37 DS2 instrument is unavailable. For control assay acceptance criteria, a result of an OD 450 < 0.100 is valid 38 for a Negative Control, whereas a result of an OD 450 > 0.500 is valid for a Positive Control. A result of an 39 OD 450 < 0.200 is considered to be negative for Salmonella . A result of an OD 450 ≥ 0.200 is considered 40 presumptive positive for Salmonella . 41 42 ) measurements
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