Total Collaborative Study Protocol_Solus One Salmonella v1 1

Appendix 10.2 Study Materials  1  2  Materials provided by Solus Scientific, Sponsor Laboratory  5  6  Materials provided by Q Laboratories, Inc., Coordinating Laboratory  7  8  1. Standard Methods Agar for background APC of NFDM   9  10  Enrichment  11  2. BPW  12  3. Brilliant Green Dye  13  4. Individual Ingredients to prepare FDA RV  14  5. TT Broth for FDA reference method  3  4  1. Solus One Salmonella test kits 

15  16  17  18  19  20  21  22  23  24  25  26  27  28  29  30  31  32 

Selective agar 

6. Hektoen Agar 

7. XLD Agar 

8. Bismuth Sulfite 

Confirmation

9. TSI  10. LIA  11. BHI 

12. API 20E or VITEK GN cards  13. O and H serotyping supplies 

Additional Supplies and Reagents required but not provided 

14. Refrigerator, capable of maintaining 2–8°C 

15. Incubator, capable of maintaining 37 ± 1°C and 41.5 ± 1°C  16. Circulating water bath, capable of maintaining 42–50°C  33  17. Heating block or water bath, capable of maintaining 85–100°C  34  18. Autoclave for decontamination of samples  35  19. Homogenizer  36  20. Measuring cylinder ,  capable of measuring 2 L  37  21. Vessel or container ,  capable of storing 2 L  38  22. Serological Pipette Bulbs (Automatic Pipette) ,  for sampling and delivering of 1–10 mL  39  23. Serological pipettes , a erosol resistant  40  24. Precision pipettes , f or sampling and delivering of 100–300 µL, and 200–1,000 µL  41

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