Total Collaborative Study Protocol_Solus One Salmonella v1 1

Romaine lettuce (bagged), NFDM (powder) and selected environmental surfaces. In addition, 

performance is equivalent to that of U.S. Department of Agriculture Food Safety and Inspection Service 

Microbiology Laboratory Manual , (MLG) 4.09 (January 2017)  Isolation and Identification of Salmonella 

from Meat, Poultry, Pasteurized Egg, and Siluriformes (Fish) Products and Carcass and Environmental 

Sponges  (2) for detection of  Salmonella  spp. from raw beef (trim) and pasteurized eggs. 

Definitions 

( a )  Probability of Detection.— Probability of Detection (POD) is t he proportion of positive  analytical outcomes for a qualitative method for a given matrix at a given analyte level or concentration. 

POD is concentration dependent.  

( b )  Difference of Probabilities of Detection .— Difference of probabilities of Detection (dPOD) is the  difference between any two POD values.  If the confidence interval of a dPOD does not contain zero, 

then the difference is statistically significant at the 5% level (3).

Principle of the Method 

Solus One  Salmonella  is an antibody‐based high sensitivity ELISA method paired with media and our 

proprietary media supplement – Solus One  Salmonella  supplement; for the rapid and specific detection 

of  Salmonella  species in select foods and environmental samples. Solus One  Salmonella  relies on 

antibodies attached to the wells of microplate strips by non‐covalent biological interactions that are 

highly specific to  Salmonella antigens. Samples are heat treated and an aliquot is added to the antibody 

coated wells.

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