Total Collaborative Study Protocol_Solus One Salmonella v1 1

Test Kit Components 

( a )  One or Five x 96 well microplates (in breakable strip format) .— Wells are coated with 

antibodies against  Salmonella  species. Store at 2–8°C. 

( b )  Negative Control (Green Label) .— 3 mL or 10 mL in working dilution. Contains diluent with 

preservative. Store at 2–8°C. 

( c )  Positive Control (Red Label) .— 3 mL or 10 mL in working dilution. Contains heat‐killed 

Salmonella Agona in diluent with preservative. The positive control is black in color. Store at 2–8°C. 

( d )  Conjugate (Orange Label) .— 11 mL or 60 mL in working dilution. Contains horseradish 

peroxidase‐antibody conjugate in diluent with preservative. Store at 2–8°C. 

( e )  Substrate (Blue Label) .— 11 mL or 60 mL in working dilution. Contains 3,3’,5,5’‐

Tetramethylbenzidine (TMB), hydrogen peroxide and stabilizers. Note: Solution should be colorless. 

Store at 2–8°C. 

( f )  Stop Solution (Silver Label) .— 11 mL or 60 mL in working dilution and contains 0.2M sulfuric 

acid. Store at 2–8°C. 

( g )  Washing Buffer Concentrate (25x) .— 6 x 10 mL or 5 x 60 mL. Store at 2–8°C. 

( h )  Washing Buffer Activator .— 6 x 1 Sachet or 5 x 1 Sachet. Store at 2–8°C. 

( i ) 

Product Instructions . 

Additional supplies and reagents  

( a )  Dehydrated Buffered Peptone Water (BPW) Medium according to ISO 6579, CAT No. MED017 .  ( b )  Solus One Salmonella Supplement , CAT No. SALSUPP‐22.5 & SALSUPP‐112 .

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