Total Collaborative Study Protocol_Solus One Salmonella v1 1

( c )  Dynex Technologies DS2 Instrument (Dynex DS2) . — Dynex Technologies, Chantilly, VA. 

( d )  Computer with DS2 Software .  ( e )  Dynex Technologies 300 µL Sample Tips .  ( f ) Dynex Technologies 1300 µL Reagent Tips . 

( g ) Sample Tubes .— 12 mm external diameter and 75 mm height. 

( h )  Polypropylene Tubes .— 2 mL and 15 mL and 25 mL. 

( i ) Frit filters .— Optional for matrices with a lot of particulate , CAT No. FRI001. 

( j ) 70% v:v Ethanol .— For preparation of the Solus One  Salmonella Supplement. 

( k )

Deionized water .

( l ) Sterile collection sponge for sampling environmental surfaces .— World Bioproducts EZ Reach 

Sponge Sampler with Hi Cap Neutralizing Buffer, or equivalent.

( m )  Sterile collection swab for sample environmental surfaces . ( n )  Letheen Broth neutralizing buffer .

Apparatus 

( a )  Refrigerator .— capable of maintaining 2–8°C. 

( b )  Incubators .— capable of maintaining 37 ± 1°C and 41.5 ± 1°C. 

( c )  Circulating water bath .— capable of maintaining 42–50°C. 

( d )  Heating block or water bath .— capable of maintaining 85–100°C. 

( e )  Autoclave for decontamination of samples .  ( f )  Measuring cylinder .— capable of measuring 2 L.

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