Total Collaborative Study Protocol_Solus One Salmonella v1 1

( g )  Vessel or container .— capable of storing 2 L. 

( h )  Filter laboratory blender bags .  ( i )  Sterile collection sponge and swabs for sampling environmental surfaces . 

( j )  Serological Pipette Bulbs (Automatic Pipette) .— For sampling and delivering of 1–10 mL. 

( k )  Serological pipettes .— Aerosol resistant. 

Precision pipettes .— For sampling and delivering of 100 µL. 

( l ) 

( m )  Micropipette tips .— Aerosol resistant. 

( n )  Multi‐channel pipette .— Capable of 100–300 µL. 

( o )  Microplate washer .  ( p )  Microplate reader with 450nm filter .  ( q )  Calibrated Thermometer .— Capable of maintaining a temperature of 37 ± 1°C, 41.5 ± 1°C, and 

85–100°C. 

( r )  Vortex mixer .— For swab homogenization. 

( s )  Calibrated Timer .  ( t )  Marker . 

Reference Materials 

Organisms used in the method comparison, robustness and stability (lot‐to‐lot) studies were 

obtained from the following sources: 

( a )  American Type Culture Collection (ATCC) .— Manassas, VA, 20110, USA. 

www.atcc.org.

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