Total Collaborative Study Protocol_Solus One Salmonella v1 1

( b )  The National Collection of Type Cultures (NCTC) .— Salisbury, SP4 0JG, UK. 

http://www.phe‐culturecollections.org.uk/collections/nctc.isp.

( c )  U.S. Food and Drug Administration Culture Collection (FDA) .— 10903 New Hampshire Avenue, 

Silver Spring, MD, 20993, USA. 

https://www.fda.gov

( d )  University of Pennsylvania Culture Collection (STs) .— Department of Microbiology, Perelman 

School of Medicine, University of Pennsylvania, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, 

PA, 19104, USA. 

( e )  Q Laboratories, Inc. Culture Collection (QL) .— Patrick Bird, Project Leader, Q Laboratories, Inc., 

Research and Development, Cincinnati, OH, 45214, USA. 

Safety Precautions  

Solus One Salmonella Method .— Ensure that the heating block reaches a temperature of 85–100°C 

and the sample is heated for 15–20 min thus ensuring the organisms are killed and the sample is safe to 

handle. The Stop Solution contains sulfuric acid which is corrosive. Wash immediately with large 

quantities of water if the solution comes into contact with skin or mucous membranes. All other Solus 

One  Salmonella  kit components are non‐hazardous. However, product usage should follow good 

laboratory practices and all disposable materials must be discarded according to appropriate waste 

procedures used in the laboratory. Protective clothing should be worn including laboratory coat, safety 

glasses, mask, and gloves where appropriate. Avoid contact with the skin.

Enrichment .— Salmonella  is a Biosafety Level 2 organism. Biological samples such as enrichments 

have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or

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