Total Collaborative Study Protocol_Solus One Salmonella v1 1

Medium supplemented with Solus  Salmonella  supplement [4.44 mL Solus  Salmonella  supplement per 

1,000 mL Solus BPW (ISO)] to 37 ± 1°C for at least 2 h prior to its addition to the sampled sponge in the 

sampling bag. Homogenize by hand massaging for 2 min ± 30 s and incubate at 41.5 ± 1°C for 16–20 h. 

( f )  Plastic environmental surface For 1” x 1” surface area, sample surface with sterile sampling swab (e.g. sponge or foam pre‐

moistened with Letheen broth). Follow sampling device manufacturers’ instructions for correct use, 

storage and transport. Pre‐warm 10 mL of Solus BPW (ISO) Medium supplemented with Solus 

Salmonella  supplement [4.44 mL Solus  Salmonella  supplement per 1,000 mL Solus BPW (ISO)] to 37 ± 

1°C for at least 2 h prior to its addition to the sampled swab in the sampling tube. Homogenize by vortex 

mixing for 2 min and incubated at 41.5 ± 1°C for 16–20 h. 

( g )  Following the incubation, gently agitate each bag or tube and transfer a 1 mL aliquot to a 

polypropylene test tube. Heat tubes at a temperature of 85–100°C for 15 – 20 min. 

( h ) After heat treatment, cool the samples to room temperature (15–25°C). This may be 

accelerated by placing the test tubes in cold water for 5 min. 

( i )  Where appropriate use frit filters with denatured matrices that contain high concentrations of 

particulates to minimize sample handling errors. 

( j )  Keep the remaining non‐heat treated samples for verification until ELISA results are obtained. 

These samples can be stored at 41.5 ± 1°C if the ELISA test is to be carried out within 2 h or at 2–8°C for 

up to 72 h if not.  

Solus One Salmonella (Manual Method) 

( a ) Remove the kit from storage at 2–8°C an hour before use to allow the components to reach

Page 14 of 47