Total Collaborative Study Protocol_Solus One Salmonella v1 1

each well, including the blank. Upon completion of the addition of the TMB Substrate, incubate the 

plate (containing the strips) for 30 ± 5 min at room temperature (15–25°C). 

( k )  After incubation, stop the reaction by adding 0.1 mL of Stop Solution to all wells, including the  “blank” well. Note: The Stop Solution will cause any blue color in wells to change to yellow.  ( l )  Where applicable read the optical densities within 10 min in a microplate reader using a 450 

nm filter (do not use reference filter), or on the Dynex DS2 instrument using the “Plate Read Only” 

setting. Inspect the wells before reading for air bubbles and if present burst with a sterile needle. Zero 

the reader against the “blank” well before the other wells are read. 

Solus One Salmonella (Automated Sample Preparation Method) 

( a ) Prior to analysis, bring the entire contents of the kit to room temperature (15–25°C) by placing  the kit on a sanitized bench top for 1 h prior to use.  ( b ) Determine the number of wells required for the test. Take the required number of strips from  the pouch and fit them to the frame provided. Note: Unused strips should be returned to the pouch and 

stored at 2–8°C. 

Analysis 

( a )  Prepare equipment

1) Turn on power to the Dynex DS2 instrument, followed by the power to the computer. 

Open the Matrix software on the computer and enter login information. The Dynex DS2 

instrument will perform a self‐diagnostic check.

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