Total Collaborative Study Protocol_Solus One Salmonella v1 1
( b ) Create a Run File
1) In the DS‐ Matrix software, select “File”, “Worklist Editor”. Then select “New Sample
Batch”.
2) Enter your sample batch ID as required.
3) Enter the total number of samples in the batch.
4) Sample ID may then be typed into the empty positions in the rack or assigned
automatically using an onboard barcode scanner.
5) If using an onboard barcode scanner slide the sample tube racks onto the machine and
click next.
6) Click on the Solus One Salmonella assay. Then click the “Add Assay” button.
7) Select “Done” when assay selection is completed.
8) Select the “Accept” button to start the run.
9) After the timeline has been accepted, loading of sample tube racks, reagents and
consumables is initiated.
( c )
Loading the Dynex DS2 Instrument
1) If not already onboard, load sample test tube racks into the slots.
2) Remove the lid from the pipette boxes and load into the instrument.
3) Load the Conjugate, TMB Substrate, and Stop Solution into the correct locations specified
by the instrument.
4) Load the Negative and Positive Controls in each position specified by the instrument.
5) Prepare and dispense the correct amount of Activated Washing Buffer.
6) Verify all disposable containers do not need emptying.
( d ) Remove Samples
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