Total Collaborative Study Protocol_Solus One Salmonella v1 1
1) When the run is completed, open the lid to the Dynex DS2 instrument and remove the
loading trays, with the sample test tubes, by pulling the loading trays out away from the
instrument. Discard sample test tubes in the proper location according to laboratory
procedures.
2) Discard the reagent tubes, negative controls, and positive controls in the proper location
according to laboratory procedures. Note: Reagents may be capped and refrigerated at 2–
8°C with the rest of the remaining kit.
Interpretation and Test Result Report
( a ) Viewing results
1) To view a report on a completed run, select the desired result file in the “Recent Test” Box,
and select the “Report” button.
2) The report can be viewed and printed from the report display screen.
3) The results are displayed in a 96 well format with each individual optical density reading.
4) A secondary file which can be used to export the results into a LIMS system is created in a
folder on the desk top called “ELISA Export Results”, this is in a CSV (comma separated
values) file format.
Immunoassay result interpretation
The presence or absence of the pathogen is expressed as optical density (OD 450
) measurements
using the Dynex DS2 instrument plate reader or a microplate reader with a 450 nm filter where a Dynex
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