Total Collaborative Study Protocol_Solus One Salmonella v1 1

( e )  Autoclave for decontamination of samples . 

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( f )  Homogenizer 

(g)       Measuring cylinder .— capable of measuring 2 L.  ( h )  Vessel or container .— capable of storing 2 L. 

( i ) 

Filter laboratory blender bags . 

( j )  Sterile collection sponge and swabs for sampling environmental surfaces .  6  ( k )  Serological Pipette Bulbs (Automatic Pipette) .— For sampling and delivering of 1–10 mL.  7  ( l )  Serological pipettes .— Aerosol resistant.  8  ( m )  Precision pipettes .— For sampling and delivering of 100–300 µL, and 200–1,000 µL.  9  ( n )  Micropipette tips .— Aerosol resistant.  10  ( o )  Multi‐channel pipette .— Capable of delivering 100–300 µL.  11  ( p )  Microplate washer .  12  ( q )  Microplate reader with 450nm filter .  13  ( r )  Calibrated Thermometers .— Capable of measuring a temperature of 37 ± 1°C, 41.5 ± 1°C, and  14  85–100°C.  15  ( s )  Vortex mixer .— For swab homogenization.  16  ( t )  Calibrated Timer .  17  ( u )  Marker . 

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C.  Media and Reagents 

( a )  One or Five x 96 well microplates (in breakable strip format) .— Wells are coated with 

antibodies against  Salmonella  species. Store at 2–8°C. 

( b )  Negative Control (Green Label) .— 3 mL or 10 mL in working dilution. Contains diluent with 

preservative. Store at 2–8°C. 

( c )  Positive Control (Red Label) .— 3 mL or 10 mL in working dilution. Contains heat‐killed  26  Salmonella Agona in diluent with preservative. The positive control is black in color. Store at 2–8°C.  27  ( d )  Conjugate (Orange Label) .— 11 mL or 60 mL in working dilution. Contains horseradish  28  peroxidase‐antibody conjugate in diluent with preservative. Store at 2–8°C.  29  ( e )  Substrate (Blue Label) .— 11 mL or 60 mL in working dilution. Contains 3,3’,5,5’‐ 30  Tetramethylbenzidine (TMB), hydrogen peroxide and stabilizers. Note: Solution should be colorless.  31  Store at 2–8°C.  32  ( f )  Stop Solution (Silver Label) .— 11 mL or 60 mL in working dilution and contains 0.2M sulfuric  33  acid. Store at 2–8°C.  34  ( g )  Washing Buffer Concentrate (25x) .— 6 x 10 mL or 5 x 60 mL. Store at 2–8°C.  35  ( h )  Washing Buffer Activator .— 6 x 1 Sachet or 5 x 1 Sachet. Store at 2–8°C.  36  ( i )  Product Instructions . 

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D.  Additional supplies and reagents  

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