VITEK MS Collaborative Study Outline - eBook

VITEK MS Collaborative Study

June 2019

completely. 5.8.5.10 Once the spot is completely dry, 1.0 μl of the VITEK MS-CHCA is added to each spot. 5.8.5.11 When the VITEK MS-DS target slides have been prepared, they must be tested within 72 hours. Prepared slides should be stored at room temperature in its original packaging until they are tested. 5.8.6 Analysis on the VITEK MS Instrument 5.8.6.1 Follow VITEK MS package insert instructions for running the instrument. 5.8.6.2 Log onto the software, verify your VITEK MS-DS is dry and open the instrument drawer using the computer software. 5.8.6.3 Place the VITEK MS-DS into the adapter and scan the barcode. The application will display the spot positions of the test isolates. 5.8.6.4 Place the adaptor into the carrier, face up with angled corners to the front, barcodes on the left side. 5.8.6.5 Slide the adapter into the carrier until it cannot go any further and start the application. 5.8.6.6 When acquisition is complete, remove the VITEK MS-DS target slide. 5.8.7 Interpretation and Test Result Report 5.8.7.1 The VITEK MS system identifies organisms by comparing the characteristics of the obtained spectrum to the VITEK MS knowledge base. A percent agreement calculation is provided to the end user. 5.8.7.2 A quantitative value, the confidence value, is calculated. and expressed the similarity between the unknown organism and every organism or organism group of the knowledge base. Thresholds are applied in order to retain only the more significant organisms or organism groups. 5.8.7.2.1 A single identification is displayed with confidence value between 60 to 99.9 when one significant organism or organism group are identified. 5.8.7.2.2 Low Discrimination identifications are displayed when more than one significant organism or organism group are identified, but not more than 4 in total. In this case, the sum of confidence values is equal to 100.

5.8.7.2.3

When more than 4 organisms or organism groups are identified, the organism is considered as non-identified. In this case, a list of possible organisms is displayed, and the sum of confidence values is less than 100. When no match is found, the organism is considered as non- identified.

5.8.7.2.4

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