VITEK MS Collaborative Study Outline - eBook

VITEK MS Collaborative Study

June 2019

packaging and components are intact. Allow the VITEK MS-DS target slides to come to room temperature before use.

Test Portion Analysis For each panel, all collaborators shall initiate the analysis according to the collaborator outline. Refer to the Study Flowchart in Appendix 8.4 for an overview of the method. 1. Culture Preparation 1.1 For each isolate, use a sterile loop to streak growth onto the appropriate agar. See Table 1 for a list of agars and incubation time periods for each panel. Observe plates each day for growth. Testing can be initiated when growth on the plates is observed. Note * - Growth may or may not be present on each agar plate during the incubation time period. In instances where growth appears on multiple agar plates for the same isolate, testing should be performed from each plate. If no growth is present, mark as no growth on the data sheet. Table 1: Panel Agar Plates and Incubation Condition Panel Agar Incubation Conditions

Tryptic Soy Agar with 5% Sheep Blood (TSAB), Modified Oxford Agar (MOX) Agar Listeria according to Ottavani and Agosti (ALOA) BACARA CHROMID S. aureus Elite agar (SAIDE)

18-72 h 35 o C

Panel 1

TSAB, ASAP, Xylose Lysine Deoxycholate (XLD), CHROMID Salmonella, Enterobacter Selective Isolation Agar (ESIA) CHROMID EHEC

18-72 h 35 o C

Panel 2

2 – 7 days 25 o C

Panel 3

Sabouraud Dextrose Agar (SDA)

2. VITEK MS Microbial Identification Method 2.1 General Preparation

2.1.1 Sample preparation is done in conjunction with the VITEK MS Prep Station. Review the VITEK MS Prep Station section of the User’s Manual before preparing the samples. Scan each VITEK MS-DS and use the keyboard to fill in the slide information. 2.2 Quality Control 2.2.1 Perform quality control analysis as outlined in the VITEK MS user manual or according to your standard laboratory procedures. 2.2.2 A negative control check, VITEK MS-CCHA with no bacteria, should be analyzed with each set of testing. 2.3 Sample Preparation for Bacteria NOTES: Powder-free gloves should be used when handling VITEK MS-DS target slides. Avoid touching the spots on the slide. Handle the slide by the end of the slide. Do not write or mark on the slide.

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