VITEK MS Collaborative Study Outline - eBook

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(c) VITEK MS‐DS target slides (Ref. 410893)  (d) API suspension medium (Ref. 70700/70640/20150)  

(e) Acetonitrile 

(f) Ethanol (99.8%, 96% or absolute) 

Apparatus 

(a) Calibrated loops (1μL) 

(b) Precision micropipette (0.5‐2μL) 

(c) Sterile colorless pipette tips without filter (0.5‐2μL) 

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(d) Eppendorf tube (2 mL) 

Standard Reference Materials 

(a) Calibration strain: Escherichia coli ATCC 8739 

Safety Precautions   15  All samples, microbial cultures and inoculated products should be considered infectious and handled  16  appropriately. Aseptic technique and usual precautions for handling the bacterial group studied should  17  be observed throughout this procedure. Refer to "CLSI M29‐A, Protection of Laboratory Workers From  18  Occupationally Acquired Infections; Approved Guideline ‐ Current revision". For additional handling  19  precautions, refer to "Biosafety in Microbiological and Biomedical Laboratories ‐ CDC/NIH ‐ Latest  20  edition", or to the regulations currently in use in each country.  21  Do not use VITEK MS‐DS target slides after the expiration date. Before use, check that the packaging and  22  components are intact. Allow the VITEK MS‐DS target slides to come to room temperature before use. 

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General Preparation  25  Sample preparation is done in conjunction with the VITEK MS Prep Station. Review the VITEK MS Prep  26  Station section of the User’s Manual before preparing the samples.  

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Sample Preparation for Bacteria  29  NOTES: Powder‐free gloves should be used when handling VITEK MS‐DS target slides. Avoid touching the  30  spots on the slide. Handle the slide by the end of the slide. Do not write or mark on the slide.  31  Before adding samples to the VITEK MS‐DS target slide, place the VITEK MS‐DS target slide on a flat  32  surface, such as a silicone mat, that will prevent it from sliding.  33  The VITEK MS‐DS target slide used for the VITEK MS is the disposable VITEK MS‐DS target slide with 48  34  sample positions. This VITEK MS‐DS target slide is for single‐use only. The sample positions are divided  35  into three sections. Each section is called an acquisition group. The three small spots in the center of  36  each acquisition group must not be used for identification of microorganisms. These three small spots  37  are used for calibration 38

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