VITEK MS Collaborative Study Outline - eBook

VITEK MS Collaborative Study

June 2019

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Aerococcus viridans

Citrobacter freundii Cronobacter sakazakii Enterobacter aerogenes Escherichia coli O157:H7 Escherichia coli O26 Klebsiella pneumonia Escherichia coli

Candida glabrata Candida tropicalis

Bacillus cereus

Enterococcus faecalis Enterococcus faecium

Cryptococcus neoformans Geotrichum capitatum

Listeria innocua Listeria ivanovii

Kodamaea ohmeri Malassezia furfur

Listeria monocytogenes Micrococcus luteus Staphylococcus aureus

Rhodotorula mucilaginosa Saccharomyces cerevisiae Trichosporon mucoides Aspergillus fumigatus Penicillium chrysogenum Fusarium proliferatum ,

Proteus mirabilis

Pseudomonas fluorescens

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Staphylococcus haemolyticus Staphylococcus hominis Streptococcus pneumonia

Salmonella Newport

Salmonella Typhimurium

Vibrio vulnificus

4.3 Shipment of Test Portions 4.3.1 Test portions will be packaged in leak-proof insulated containers and shipped (according to the Dangerous Goods Regulations IATA for Infections Substances) by overnight. 5 VITEK MS Microbial Identification Method 5.1 Applicability Applicable for the identification and confirmation of Gram-positive and Gram-negative (aerobic and anaerobic) and fungal isolates 5.2 Test Kit Information 5.2.1 VITEK ® MS.—bioMérieux, Inc., REF 411071

5.3 Additional supplies and reagents required but not provided 5.3.1 VITEK MS-CHCA matrix.—bioMérieux, Inc., REF 411071 5.3.2 VITEK MS-FA.—bioMérieux, Inc., REF 411072 5.3.3 VITEK MS-DS target slides.—bioMérieux, Inc., REF 410893 5.3.4 VITEK MS Mould Kit.—bioMérieux, Inc., 415680 5.3.5 API suspension medium.—bioMérieux, Inc., REF 70700/70640/20150 5.3.6 Acetonitrile 5.3.7 Ethanol (99.8%, 96% or absolute)

5.4 Apparatus and equipment

5.4.1 Calibrated loops.― 1μL 5.4.2 Precision micropipette.―Capable of sampling and delivering 0.5-2μL 5.4.3 Sterile colorless pipette tips without filter.―Capable of sampling and delivering 0.5-2μL 5.4.4 Eppendorf tube.―Capable of sampling and delivering 2 mL

5.4.5 Sterile cotton swabs 5.4.6 Vortex-type mixer

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