Working Group Chair Presentation Book

Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted Formatted

... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...

80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99

6. System suitability testsand/oranalyticalquality control:

Suitablemethodswill includeblank check samples,and check standards at the lowestpoint

andmidrangepointof theanalytical range.

7. Potential ReferenceMaterial(s):

WholeEgg

x NIST8445

x LGCSAL-RSM-5 (Check for characterization level)

NonfatMilkPowder

x NISTSRM1549 (nonfatmilkpowder) x NISTSRM1549a (wholemilkpowder)

Peanut

NISTSRM2387 (peanutbutter)

x

x LGC FAL-RFM1017-XXX

Hazelnut

x Noknown referencematerialavailable.

100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130

x LGC FAL-RFM1015-50orFAL-RFM1015-50ÂorFAL-RFM1015-5

Additionalmaterials canbe foundat the LGCandFAPASwebsites

LGC= http://www.lgcstandards.com/HK/en/search/?q=allergen:relevance:category:CEFP_77243 FAPAS= http://fapas.com/quality-control-materials/Available-quality-control-materials.cfm Refer toAnnexF: DevelopmentandUseof In-HouseReferenceMaterials inAppendixF: Guidelines forStandardMethodPerformanceRequirements ,19 th Editionof theAOAC

INTERNATIONALOfficialMethodsofAnalysis (2012). Availableat:

http://www.eoma.aoac.org/app_f.pdf

NIST=National Institute forStandardsandTechnology.

LGC= formerly theUK Laboratoryof theGovernmentChemist ,now simply “LGCStandards”. FAPAS= formerly the FoodAnalysis PerformanceAssessmentScheme in theUK, now simply

“FAPAS”.

8. ValidationGuidance :

Methoddevelopers shall submit LOQ,MDL, recoveryandprecision data evaluating for the

matrices inTable2.

AppendixD:Guidelines forCollaborativeStudyProceduresToValidateCharacteristicsofa MethodofAnalysis;19 th Editionof theAOAC INTERNATIONALOfficialMethodsofAnalysis

(2012). Availableat: http://www.eoma.aoac.org/app_d.pdf

AppendixF: Guidelines forStandardMethodPerformanceRequirements;19 th Editionof the

AOAC INTERNATIONALOfficialMethodsofAnalysis (2012).Availableat:

http://www.eoma.aoac.org/app_f.pdf

9. MaximumTime-To-Result: None

13

131 132 133

Table1:Methodperformance requirements.

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Targetallergen

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Parameter

wholeegg

milk

peanut

hazelnut

Analytical Range (ppm)

10-1000

10-1000

10-1000

10-1000

LOQ (ppm*)

5

10

10

10

MDL (ppm*)

101.65

103

103

103

Recovery (%)

60-120%

60-120%

60-120%

60-120%

%RSD

чϮϬй

чϮϬй

чϮϬй

чϮϬй

r

%RSD

чϯϬй

чϯϬй

чϯϬй

чϯϬй

R

*Reportedasppmof the targetallergen in food commodity, i.e.,25ppmof “wholeegg” in cookies.

Definitions for LOQandMDLprovided in section4.

134 135 136 137 138 139 140 141 142 143 144 145 146 147 148

14