the Stakeholder Panel on Dietary Supplements

Official Methods

• United States Pharmacopeia – Echinacea angustifolia root, powdered root, and powdered  extract: HPLC‐UV for phenolic compounds – Echinacea pallida  root, powdered root, and powdered  extract: HPLC‐UV for phenolic compounds  – Echinacea purpurea root, powdered root, and powdered  extract: HPLC‐UV for phenolic compounds • European Pharmacopoeia – Echinacea angustifolia root (whole or cut): HPLC‐UV for  phenolic compounds  – Echinacea pallida  root (whole or cut): HPLC‐UV for phenolic  compounds  – Echinacea purpurea root (whole or cut): HPLC‐UV for  phenolic compounds – Echinacea purpurea dried herb (whole or cut): HPLC‐UV for  phenolic compounds • Amounts of individual phenolics vary substantially  among species and plant parts: method  performance criteria will depend on concentration • Chlorogenic acid ‐ if present – is found at low  concentrations: larger amounts may indicate  addition of extraneous material • Echinacea products combined with other plant  extracts will require additional validation (e.g.,  selectivity) • Analyte standards and vouchered botanical  materials are commercially available (no NIST  standards) SMPR Key Points