the Stakeholder Panel on Dietary Supplements

General Analytical Needs

▪ Individual quantitation of six major kava kavalactones in raw materials, ingredients, and finished products.

▪ Quantitation of flavokavain B?

▪ Ability to differentiate noble from two-day cultivars?

Existing Kavalactone Methods

▪ Spectrophotometric ▪ 1g root/rhizome 3 ml acetone ▪ Dominate wavelength said to differentiate noble, two day, and mixtures of the two. ▪ ≤ 577.5 nm for noble kava ▪ ≥ 581.0 nm for non-noble kava

▪ Premised on unknown constituent differences ▪ Possibly chalcones

From left to right, acetonic extracts of noble (test tubes 1–4), two-days (5– 9) and wichmannii varieties (10–12).