2016 OMB Summer Meeting

AOAC OMB Meeting Book

394

AOAC 2012.22 (FINAL VERSION REVIEW) - JUNE 2016 FOR ERP USE ONLY DO NOT DISTRIBUTE

(e). Identification .- Identify the ascorbic acid peak in the chromatograms of the

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sample solutions by comparison with the retention time and UV spectrum of

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the corresponding peak in the standard solution.

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H.

Calculations

Calculate the concentration of vitamin C, in mg ascorbic acid/100 g expressed in product “as 104

is” for RTF (ready to feed) products or as “reconstituted powder” for powder samples, as 105

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follows:

100 x x V x V I)- (A

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1

3

C



1000 x x Vm x S

2

where A = response (height or area) of the ascorbic acid peak obtained for the sample 108

solution, m = weight of the test portion, in g (2.0 g of product as is or reconstituted powder), 109

V 1 = volume of the test solution (volume used to dissolve the test portion) in mL (10 mL), V 2 110

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= volume used in the sample dilution (1.0 mL), V 3

= volume of the final sample dilution (10

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mL).

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Note: if results expressed in powder sample are needed, use the reconstitution rate to 114

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calculate (c *225/25).