AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

1138 multi-class pesticide residues in fruits and vegetables. Each participating laboratory will conduct two-phase studies: pre- collaborative and collaborative study. We will send standard solutions, blank samples and aged samples to each participating laboratory. We are planning to organize this AOAC collabora- tive study in later 2019, and intend to further develop this HRMS technique to be an AOAC Official Method. Experts who are interested are kindly welcomed to join us. Presenter: Guofang Pang, Chinese Academy of Inspection and Quarantine, Beijing, China, Email: ciqpang@163.com P-W-059 Wendy Andersen , Joseph Storey , Sherri Turnipseed , Christine Casey , U.S. Food and Drug Administration, Denver, CO, USA Amoxicillin and Ampicillin Residues in Fish Penicillin antibiotics can be effective in the treatment of bacte- rial infections in aquacultured fish. While penicillins are not approved for fish treatment in the United States, amoxicillin and ampicillin are permitted for use in other countries. Penicillins are more polar than other veterinary drugs, and they lack stability under common extraction conditions. Amoxicillin is not well-extracted from tissues using organic solvents, but matrix interference can be problematic for low concentration resi- due quantification when aqueous extractions are performed. Quantitative tissue analysis for amoxicillin can require laborious procedures with extensive protein precipitation and sample clean-up steps. For fish tissues, amoxicillin metabolites have been identified following dosing, and may be useful marker residues to indicate amoxicillin use. The objective of this study is to develop a relatively simple quantitative regulatory method for low residue level analysis of amoxicillin, ampicillin, and relevant metabolites in fish tissue. Differential ion mobility spectrometry was explored to increase selectivity of residue detection with minimal extract purification procedures. Presenter: Wendy Andersen, U.S. Food and Drug Administration, Denver, CO, USA, Email: wendy.andersen@fda.hhs.gov P-W-060 Gabrielle Anderson , R. Lucas Gray , Brooke Roman , Ben Strong , Ron Sarver , Robert Donofrio , Neogen Corp., Lansing, MI, USA Validation of BetaStar S 4D for Tetracyclines in Porcine Saliva BetaStar S 4D for Tetracyclines, a lateral flow dairy antibiotic detection kit, has been validated for detection of tetracyclines at 100 parts per billion (ppb) using porcine saliva samples with non-detectable amounts of tetracycline, 50 ppb of tetracyclines and 100 ppb of tetracyclines. After a 5 minute incubation in the saliva sample, the lateral flow device is visually interpreted by the operator. The kit’s ruggedness was tested over two days of a multi-operator, multi-lot analysis. Robustness, or intentional variation of operating parameters, was tested over one day. Robustness parameters were temperature, amount of saliva vs amount of buffer, number of drops of saliva, syringe filter stuffing volume, and a complete randomization of all samples tested.

Both lots produced 100% passing visual results for all levels when tested by multiple operators over multiple days. When an intentional variation of operating parameters was done, the 50 ppb tetracycline sample accurately produced negative results 89% of the time. All other parameters tested passed at 100%. We conclude that this is a viable screening method for tetracy- clines in porcine oral fluid. Presenter: Gabrielle Anderson, Neogen Corp., Lansing, MI, USA, Email: ganderson@neogen.com P-W-061 Uwe Oppermann , Waldemar Weber , Shimadzu Europa GmbH, Duisburg, Germany Analyzing 360 Pesticides in Less Than 10 Minutes Using Fast GC-MS/MS Contamination of food products with pesticides has been of concern for many years due to the risk of acute or delayed adverse health and environmental effects. The global use of pesticides is increasing, as is exposure to them; and imports of raw foodstuffs from unknown sources is rising too. Consequently, the number of samples in analytical instrumentation as well as pesticide monitoring has escalated significantly in the last decade. To handle this high sample load, a quick, easy and cheap cleanup procedure called QuEChERS was established some time ago. Unfortunately, samples prepared using this method contain large matrix signals, which popularised the use of highly selective tandem MS. Along with matrix interfer- ence, the analysis time is a crucial point when handling a high sample load. The usage of narrow bore capillary columns has proven to be a powerful tool for drastically reducing analysis time while maintaining chromatographic resolution in different GCMS applications. Combining the speed of fast gas chroma- tography (GC) and the selectivity of tandem mass spectrometry (MS) increases laboratory efficiency and reduces working costs. Fast MRM (Multiple Reaction Monitoring) switching modes with no interfering crosstalks are therefore needed. The potential of this approach is demonstrated by analysing 360 pesticides in QuEChERS apple extract in less than 10 minutes by using a Shimadzu GCMS-TQ8050 NX ultra-high sensitivity Triple Quadrupole system. Presenter: Uwe Oppermann, Shimadzu Europa GmbH, Duisburg, Germany, Email: uo@shimadzu.eu P-W-062 Cheng-Ting Tsai , Chia-Ling Chang , Ching-Yi Huang , Taipei City Government, Taipei, Taiwan Determination of Veterinary Drug in Pork Using UHPLC-Q-Orbitrap-HRMS Veterinary drugs are broadly used to prevent or cure disease, enhance lean meat growth, and promote or regulate physiological function. Inappropriate use of veterinary drugs can cause residues over the allowance and adverse effects to humans. The traditional analytic method for veterinary drugs may use different extraction methods or instrument and take a lot of time. In this study, a simple sample extraction procedure coupled with UHPLC-Q-Orbitrap- HRMS in Full MS/dd-MS2 mode was used to screen common

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