AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

veterinary drugs in Taiwan, including sulfonamide, quinolone and beta-lactam antibiotics. One precursor ion and one product ion were chosen to comfirm the analyte. The method can apply to routine screening for veterinary drugs in pork. Presenter: Cheng-Ting Tsai, Taipei City Government, Taipei, Taiwan, Email: blackjing@health.gov.tw P-W-063 Uwe Oppermann , Marion Egelkraut-Holtus , Shimadzu Europa GmbH, Duisburg, Germany; Jürgen Schram , Gerrit Renner , University Krefeld, Krefeld, Germany Identification of Microplastics in Food and Food Packaging Using FTIR Microscopy Concern about microplastic pollution and its impact on the environment and human health is a hot subject discussion on the global scale. Microplastics are classified in two groups, primary and secondary types. Primary microplastics are any plastic fragments or particles that are already 5.0 mm in size or less before entering the environment. They include microfibers from clothing, microbeads from cosmetics and cleaning products and plastic pellets. Secondary microplastics are microplastics that are generated from the degradation of larger plastic products once they are present in the environment and treated in natural weathering processes. Such sources of secondary microplastic include water and soft drinks bottles from PET and other materi- als, fishing nets, and plastic bags. Both types of microplastics are present in the environment at elevated levels, particularly in the sensitive marine ecosystems and furthermore as contaminants in the the production process of food and beverages. The analysis of microplastics is mainly performed using Fourier transformation infrared spectroscopy coupled with a microscope and spectral library for identification. The LabSolutions software package for FTIR includes approximately 12,000 spectra. Furthermore, the thermally degraded plastic library with another 111 spectra of unheated and heat-degraded at 200 to 400°C for 13 types of plastics has been used as an additional tool for identification of microplastics in food and beverages. Presenter: Uwe Oppermann, Shimadzu Europa GmbH, Duisburg, Germany, Email: uo@shimadzu.eu P-W-064 Theresa Sosienski , Dorothy Yang , Agilent Technologies, Inc., Santa Clara, CA, USA; Jerry Zweigenbaum , Agilent Technologies, Inc., Wilmingtoin, DE, USA Veterinary Drug Detection in Pork and Milk Using an Ultivo LC/TQ with ESI Ion Source Veterinary drugs aid in disease prevention and growth promo- tion for livestock operations. Global regulatory agencies set maximum allowable levels for many veterinary drugs in animal-derived products. The precise quantification of 12 regulated veterinary drug compounds in pork and milk are demonstrated using the Agilent Ultivo LC/TQ. The veterinary drugs were analyzed in meat and milk products obtained from local grocery stores. Samples of 2 g of pork or milk were prepared with a simple and quick extraction using acetonitrile

and water prior to analysis. Pork samples were subject to a final clean-up with Agilent Captiva EMR–Lipid cartridges. The compounds were separated in a 10 min method using a super- ficially porous EC-C8, 2.1 × 100 mm, 2.7 µm analytical column in a gradient elution with water modified with 0.2% formic acid and 0.5 mM ammonium fluoride, and acetonitrile as mobile phases. Compounds were analyzed in dynamic multiple reaction monitoring (dMRM) mode with an ESI source. The drugs were accurately quantified at ½ the maximum residue limit (MRL), as defined by global veterinary drug regulations, while most could be quantified at 1/10 MRL, the lowest level tested in this study. Excellent precision was observed at the LOD with RSD below 14% for all compounds tested. The calibration curves showed good linearity with R 2 values greater than 0.98. Recoveries ranged from 60-120% for most compounds, demonstrating the exceptional efficiency of the extraction method. Presenter: Dorothy Yang, Agilent Technologies, Inc., Santa Clara, CA, USA, Email: dan-hui-dorothy.yang@agilent.com MICROBIOLOGICAL METHODS P-W-065 Irshad Sulaiman , Nancy Miranda , Steven Simpson , U.S. Food and Drug Administration, Atlanta, GA, USA Identification of Clostridium Species by Matrix Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry and 16S rRNA Sequencing Clostridium is a genus of anaerobic, fermentative, spore-form- ing Gram-positive bacteria, that contains approximately 100 species. Of these, C. botulinum , C. perfringens , C. tetani and C. difficile are recognized human-pathogenic species, causing life-threatening botulism, diarrhea, food poisoning, and teta- nus. Identification of Clostridium spp. is vital for the detection of foodborne pathogen and source tracking of contaminated foods, and to understand the transmission dynamics of poten- tially lethal foodborne diseases in humans. MALDI-TOF MS is an emerging technology that can provide rapid and accurate bacterial identification by utilizing intact cells without extraction or purification. This efficient diagnostic tool has been extensively applied to identify various bacteria isolated from clinical, envi- ronmental, and outbreak samples. More recently, MALDI-TOF MS is used for species identification of various Gram-positive and Gram-negative bacteria causing foodborne diseases. In this study, we have analyzed a total of 49 Clostridium -like Gram-positive bacterial isolates recovered from food and envi- ronmental samples, which also included one ATCC reference C. perfringens isolate and three ATCC reference C. difficile isolates, using bioMérieux VITEKMS system. The VITEK MS identified all 49 Clostridium -like Gram-positive bacterial isolates tested. Sequencing of 16S rRNA gene was performed to confirm and compare the species-identification data generated by the VITEK MS system. Presenter: Irshad Sulaiman, U.S. Food and Drug Administration, Atlanta, GA, USA, Email: Irshad.Sulaiman@fda.hhs.gov

102 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL