AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Tuesday

collection and analysis of reference samples, development and validation of the non-targeted testing statistical models, monitor- ing and maintenance, as well as advice on abnormal samples handling. Use of the guidance, reference materials in non-tar- geted method development will be presented with examples. SYMPOSIUM: The Complexity of Validating STEC Methods to Address Varying Global Needs 8:15 AM – 9:45 AM Plaza F Chairs: DeAnn Benesh, 3M Jesse Miller, NSF International 8:20 AM European Views on STEC Method Requirements Patrice Arbault, Bioadvantage The European perspective on any microbiology testing would include both those of the European Union and individual country level views. In general ISO standards are used as the basis for STEC testing methodologies, but country requirements can also be factor in method determination. Current thoughts and approaches will be presented. 8:40 AM Criteria for FDA STEC Method Validation Julie Kase, U.S. Food and Drug Administration FDA considers the presence of particular STEC in food to be an adulterant. These E. coli are classified as high or low risk to human health by certain characteristics such as their viru- lence factors and the FDA BAM method is regularly updated as advancements have been made in their detection. The most current approach will be presented. 9:00 AM Fit-For-Purpose?: Challenges in Establishing Validation Criteria for STEC Alexander Gill, Irene Iugovaz, Health Canada Shiga toxin producing Escherichia coli are significant food borne pathogens, with high genomic and phenotypic diversity. Outbreaks have also been associated with diverse food prod- ucts. In this talk we summarize the factors which can complicate the design of validation studies for STEC and discuss potential approaches. 9:20 AM

introduction of the MicroVal validation platform, it’s processes and partners. We will explain how we validate and certify meth- ods, especially when they need that extra flexibility. SYMPOSIUM: New Blood 2019—Developing Methods for the Detection of Important Chemical Analytes, Residues and Contaminants 10:15 AM – 11:45 AM Grand Ballroom 1 Chairs: Alexander Krynitsky, Symbiotic Research Michael McLaughlin, U.S. Food and Drug Administration Jon Wong, U.S. Food and Drug Administration 10:20 AM Simultaneous Targeted Quantification and Suspect Screening of Environmental Contaminates and Pesticide Residue in Food by High-Resolution LC-QTOF James Pyke, Karen Yannell, Kai Chen, Agilent Technologies, Inc. Governments are seeking to monitor a larger range of pesticides in our food. Logistical management of traditional targeted tech- niques becomes exponentially harder as compounds are added. Some food safety laboratories manage the cumulative burden by routinely quantitating only commonly detected pesticides, then screening a larger number of pesticides with triggered MRM techniques on triple quadrupole (TQ) technology. However, TQ methods require initial development, ongoing maintenance and as the data is targeted, retrospective analysis is impossible. A workflow that simultaneously quantitates pesticides while acquir- ing non-targeted data for suspect screening is described. A new high-resolution (30,000 for 118 m/z ) quadrupole time-of-flight and new suspect screening software integrated with quantita- tion software was employed. To exemplify the workflow, we prepared various foods with relevant QuEChERS protocols and quantitated 195 pesticides with five calibrators (5-100 ppb). Another 182 pesticides were monitored in the same workflow, which could grow retrospectively. The samples were measured using typical reverse-phase chromatography in positive ioniza- tion in various acquisition modes: AllIons, quadrupole resolved AllIons (Q-RAI) and Auto MS/MS for comparison. We show good reproducibility, low levels of quantitation, and good linearity for target analytes. Quality control samples spiked with ‘suspect’ pesticides were identified with high confidence in a seamless manner. 10:40 AM Seafood Reference Materials: Novel Production Methods and Analytical Assessment for Authentication of Food Products Debra Ellisor, Clay Davis, Steven Christopher, Melissa Phillips, Benjamin Place, Kate Rimmer, Laura Wood, Rebecca Pugh, U.S. National Institute of Standards and Technology

Validating a STEC Method at MicroVal: Flexibility in a Standardized Process Joost Witsenburg, MicroVal

With over 50 food micro methods certified, MicroVal is well experienced in ISO 16140 part 2 and part 6 validations. Still, every validation study requires flexibility and commitment - and a STEC method definitely does. This presentation will give an

14 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL