AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Tuesday

Seafood is one of the most highly traded international commod- ities. Product price is determined by characteristics potentially falsified at import (i.e., species, provenance, weight), result- ing in inflated payouts, encouraging unsustainable practices and damaging the domestic seafood economy. Aquacultured seafood differs from wild-caught in its nutritional content and the potential presence of additives, dyes, and antibiotics that can negatively impact human health. Food regulatory and customs agencies would benefit from authentic seafood materials to aid in identifying cases of import fraud. To this end, NIST has gener- ated aquacultured and wild-caught shrimp and salmon reference materials procured from verified sources to authenticate consum- able seafood products. A non-targeted MS-based approach was used to identify metabolomic and proteomic feature differ- ences between the wild-caught and aquacultured materials. These include fatty acids, aquaculture antibiotics, food additives, and pharmaceuticals. The identification of specific antibiotics and drug products present in the aquacultured seafood led to the production of a small-batch, custom amended material. Known concentrations of common aquaculture antibiotics were added to a portion of the wild-caught salmon material during the cryogenic production stage. The success of the amendment procedure and the spike stability will be discussed, as well as characterization of the materials using genetics and elemental/ isotopic profiling. 11:00 AM Optimizing GC-MS and GC-MS/MS Analysis of 3-MCPD and Glycidyl Esters Jana Rousova, Joe Konschnik, Hansjoerg Majer, Alexandria Pavkovich, Chris English, Restek Corporation 3-MCPD and glycidyl esters in edible oils are contaminants that are formed through refining processes and several of these substances have been classified as possible human carcino- gens. Methods, which are similar to one another, have been developed by ISO, AOCS, and DGF for analyzing these contaminants. While the method covers extraction and deri- vatization techniques in detail, very little attention is paid to the GC-MS methods. With emerging automated systems, it is important to simplify and speed up the method by optimizing the parameters, to include switching to split injection. The initial optimization of the temperature program led to an 8-minute decrease in the analysis time and additional time can be saved by utilizing free method development software. The employment of split injection resulted in better peak shape and achieved limits of detection that were comparable to splitless injection. Further evaluation of split injection revealed that the same performance is achieved regardless of inlet temperature resulting in greater flexibility for different inlet configurations. 11:20 AM The Detection and Identification of Tadalafil Analogues in Taiwan Hui-Chun Lee, Chia-Fen Tsai, Der-Yuan Wang, Taiwan Food and Drug Administration, Yen-Chun Huang, Super Laboratory Company Limited, Yun-Lian Lin, China Medical University Synthetic phosphodiesterase type 5 (PDE-5) inhibitors and their analogues are illegal adulterants present in enhanced sexual

performance dietary supplements. Since the identification of the first analogue, homosildenafil in 2002, the Taiwan FDA (TFDA) Laboratory have detected an additional 48 novel analogues. Dipropylaminopretadalafil and N -3-hydroxypropylnortadalafil are two recently identified tadalafil (Cialis ® ) analogues. Usually, when an unknown compound exhibits tadalafil-like signals but cannot be matched to any known tadalafil analogues in the TFDA adulterant screening procedure, additional purification is required to isolate the unknown so that high-resolution MS and NMR experiments could be performed to elucidate structure. Dipropylaminopretadalafil, which consists of two propyl group substitutions, was determined and identified and is derived from pretadalafil, an intermediate compound in (+)-tadala- fil synthesis and possesses a cleaved piperazinedione ring. N -3-hydroxypropylnortadalafil is a constitutional isomer with 2-hydroxypropylnortadalafil, which was reported in 2012 and detected by the Taiwan FDA Laboratory in 2015. With almost similar MS 2 spectra, N -3-hydroxypropylnortadalafil is distin- guishable by different R f values on developed TLC plates, and it is identified to have a different hydroxyl site on the propyl group by NMR. SYMPOSIUM: Food Fraud Detection Goes Mobile 10:15 AM – 11:45 AM Grand Ballroom 2 Chair: Bert Popping, FOCOS Food Consulting Strategically Smartphone diagnostics aim to bridge the gap between the laboratory and the general public by engaging consumers with their own health and dietary needs; promoting citizen science. As things currently stand, consumers are separated from the analytical procedure owing to lack of technical experience, knowledge and equipment and thus rely heavily on submitting samples to third parties for analysis. By using a smartphone as an analytical toolbox, consumers can take advantage of the many benefits of the technology such as: optical sensors, global positioning system (GPS), and the central processing unit (CPU) which allows the operation of a range of software apps. Additionally, smartphones are portable, affordable, and make for a familiar interface for the user. Smartphone diagnostics have been widely applied in the medical and preventative healthcare sectors and in recent years has become an integral point-of-care tool for food safety analysis. The technology has been utilized for a range of food safety tests, most interestingly towards food allergens, where a number of commercial companies have been working to develop easy-to-use tests designed for the end user. In this talk, a general overview of smartphones as mobile food analysis tools will be addressed. 10:15 AM Calling the Future: An Overview of Smartphone Biosensors for Food Safety Georgina Ross, Wageningen University Research, Gert I. J. Salentijn, Wageningen University, Laboratory of Organic Chemistry, Michel W.F. Nielen, Wageningen Food Safety Research

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