AOAC 133rd Annual Meeting - Final Program

and is not a desirable approach. We present work performed in the analysis of these pesticides using a HILIC liquid chroma- tography column and demonstrate the viability analyzing these compounds in one single HILIC run followed by the detection using tandem mass spectrometry to achieve operation efficiency and data quality. 8:40 AM Chemical Risk Assessment of Ingredients Grace Bandong, Eurofins Food Integrity and Innovation Food Safety Modernization Act (FSMA) was signed into law on January 4, 2011 and in September 2015 the FDA published the final rule on Preventive Controls for Human Food with the last compliance dates set to September 2018. This rule requires food companies to develop a written food safety plan includ- ing a hazard analysis and risk based preventive controls on known or reasonably foreseeable hazards that could affect food manufactured, processed, packed, or held at a facility. Many HACCP programs focus on microbial hazards. However, FSMA specifically identifies pesticides, drug residues, and unapproved food and color additives as reasonably foreseeable hazards to consider. Chemical contaminants can cause serious damage to customer’s health and have major effects on business. It is necessary to protect products from chemical contaminants and identify the hazards in ingredients before they can affect the final product. It is not realistic to test every lot of ingredient for every chemical contaminant including pesticides. One approach is to develop a monitoring program for the riskiest ingredients. A risk analysis model is employed to identify and weigh risk for ingre- dients. Risk analysis follows a structured approach with three distinct components: 1) Risk Assessment 2) Risk Management 3) Risk Communication. 9:00 AM Pesticide Residues in Essential Oils: Risks, Occurrences and Practical Approaches to the Analysis in a Quality Control Environment Katarzyna Banaszewski, Anna Plocicka-Okladlo, Aaron Secrist, NOW Foods Essential oils (EOs) are complex mixtures of volatile compounds obtained from plant source by either pressing or distillation. The composition of each essential oil varies due to the plant source, production process, geographical origin, and environmental influences. Besides the naturally occurring components, EOs may contain contaminants such as pesticides, which are regarded with an increasing concern. The societal pressure to manufac- ture the purest, safest natural products mandates development of methods for precise determination of pesticide residues in essential oils. The analysis of pesticides in EOs is extremely chal- lenging due to the complexity of the EOs, and the wide variety of essential oils produced worldwide. A study examining a robust sample preparation workflow for the multi-residue determination of pesticides in essential oils was conducted. A method utilizing commercially available solid-phase extraction (SPE) solution was developed and it demonstrated adequate mitigation of matrix in all evaluated essential oils. The newly developed workflow was optimized for both, GC-MS/MS and LC-MS/MS and delivered satisfactory results for the analysis of all 10 essential oil samples. This procedure can be easily implemented in a quality control

materials. These reference materials come in the form of the food allergen commodity (i.e., milk, eggs, tree nuts, etc.) as well as processed food samples with incurred allergen commodities. Both the commodity and matrix-based reference materials are necessary to establish measurement traceability, a key element of measurement quality. The design consideration for both types of materials will be discussed. Even when available, reference materials will not improve measurement quality if they are not used properly. Best practices will be discussed. Steven Moser Memorial Session: Advanced Topics in Pesticide Analytical Methods 8:15 AM – 9:45 AM Grand Ballroom 2 Chairs: Ping Wan, Indiana State Chemist Jon Wong, U.S. Food and Drug Administration 8:20 AM Determination of Glyphosate, Glufosinate, AMPA, and Quaternary Ammonium Pesticides in Aqueous Environmental Samples by Liquid Chromatography/Tandem Mass Spectrometry Paul Yang, Calibration and Validation Group Canada, Mili Chan, Matthew Robson, Xiaoming Zhao, Laura Siu, Ontario Ministry of the Environment, Andrew Kennedy, Jordy Hsiao, Agilent Technologies, Inc. Glyphosate (Gly), glufosinate (Gluf), diquat (Dq) and paraquat (Pq) are high ionic strength herbicides that are used under brand names such as Roundup, Reglone A and Cyclone. They are commonly used as broad spectrum and non-selective herbicides for agricultural, urban, and aquatic weed control programs worldwide. Gly and Dq are regulated by the United States Environmental Protection Agency (US EPA) while Gly, Dq and Pq are regulated in Ontario, Canada, under Ontario Regulation 169/03. Glyphosate, due to its unique chemical properties and high ionic strength, usually require pre- or post-column derivatization for analysis in aquatic samples. Of late, ion-pair- ing chromatography (IPC) using C-18 stationary phase, and mixed mode stationary phases such as weak anion exchange and hydrophilic interaction chromatography (HILIC) have been applied to the analysis of these pesticides. IPC analysis use triflu- oroacetic, pentafluoropropanic or heptafluorobutanic acid as an ion-pairing agent but is subjected to extensive system carry over

22 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL