AOAC 133rd Annual Meeting - Final Program

Scientific Sessions | Wednesday

environment for monitoring of pesticide residues in a wide vari- ety of essential oils. 9:20 AM Multi-Residue Screening and Confirmation of Pesticides in Animal Tissues with Fast Sample Preparation Jing Qiu, Wei Zhang, Lin Zhang, Qi Jia, Rui Weng, Yongzhong Qian, Chinese Academy of Agricultural Sciences A sensitive method for the simultaneous determination of 308 pesticides and relevant metabolites in animal tissue was developed using UHPLC-MS/MS after clean-up with a multi-functional filter (MFF) based on QuEChERS. The sample (2 g) was extracted with 10 mL mixed solution of 1% formic acid in acetonitrile and water, by vortexing for 5 minutes, then salt out with NaCl. The extracted solution was filtered directly through an MFF containing 50 mg PSA, 50 mg C18, and 150 mg MgSO 4 . The clean-up and filter procedures were integrated using the MFF to obviously improve the work efficiency and only need simple operation, compared to traditional methods. Good linearity was shown for each analyte with the correlation coeffi- cients of >0.98. The recoveries of 225 pesticides and metabolites were 70%~120% in the beef at 10 μg /kg spiked levels, All RSDs were less than 20%. The recoveries of the other 83 pesticides are 40%~70% or 120%~150%, which can be used for qualita- tive analysis. The LODs were 1.0-10.0 μg /kg. The application indicates that the developed method is more effective and faster in the monitoring of pesticide residue in animal tissue compared to the traditional analytical method. TDRM SYMPOSIUM: The Importance of Public Private Partnerships in the Development of Reference Materials 8:15 AM – 9:45 AM Plaza F Chairs: Catherine Rimmer, U.S. National Institute of Standards and Technology Charles Barber, U.S. National Institute of Standards and Technology 8:20 AM Botanical Reference Materials for Quality Assurance and Regulatory Compliance in Dietary Supplements Holly Johnson, American Herbal Products Association Reference materials are crucial elements in laboratory test- ing of botanical materials for regulatory compliance. Natural products markets are rife with innovation and as new dietary ingredients emerge as trends; availability of adequate reference materials can be a challenge in quality control testing platforms. By leveraging collaborations with industry, reference material producers can gain insights about the critical needs for specific materials. This talk will explore the role of well characterized botanical reference materials in validating methods and describe public-private collaborations that may lead to development of

new botanical reference materials, such as hemp, in a dynamic market. 8:45 AM Bioactives Testing in Food Stuffs and Dietary Supplements: Analytical Strategy for Developing and Validating a High Performance, Transferable and Reliable Flavanol and Procyanidin Assay Ugo Bussy, Nicholas Anderson, Catherine Kwik-Uribe, Mars Symbioscience Botanical bioactives are receiving increasing attention because of their potential health benefits. In our work, we used cocoa flavanol as models and described the development of a stan- dard and a methodology that addresses researchers’, consumers’, manufacturers’, and regulators’ needs. Analytical work focused on the identification and quantification of flavanol monomers and procyanidin bioactives in cocoa using high-performance liquid chromatography with fluorescence detection. This method was developed alongside an analytical reference material and validated to achieve a precise, accurate, and transferable deter- mination of flavanols and procyanidins in cocoa-based matrices. Transferability of method performances to manufacturers, researchers, and regulators highly depends on the development and distribution of a commercially available reference material. The National Institute of Standards and Technology (NIST) and Mars Symbioscience (Mars Inc.) partnered to develop and characterize a novel cocoa flavanol extract reference material to support the harmonization of cocoa flavanol testing across laboratories. Using this newly developed methodology and standard, cocoa flavanol and procyanidin contents ranging from 3 to 500 mg/g were determined in commercial samples and discrimination was established based on cocoa flavanol relative distribution only, demonstrating the applicability of the method for both identification and quantification of flavanol and procy- anidins in cocoa derived products. 9:10 AM Infant Formula Reference Materials: A 25-Year Partnership Melissa Phillips, U.S. National Institute of Standards and Technology Because infant formula is the sole source of nutrition for develop- ing infants, infant formula is the most regulated food produced in the U.S. and worldwide. In the US, the Infant Formula Act of 1980 requires that various nutrient levels fall within a specified range or above a specified minimum. Consequently, a reference material with values assigned for these nutrients is critical to allow manufacturers to monitor their products and ensure that their analytical methods are accurate. Since the early 1990s, NIST has partnered with infant formula manufacturers and testing laboratories to understand the needs of this unique measurement community and to develop highly impactful reference materials. NIST, through partnership with an infant formula manufacturer, has provided a Certified Reference Material for traditional milk-based infant formula/adult nutritional hybrid products since 1996, and will soon characterize the fourth material in this series. In 2018, NIST released a soy and whey protein-based

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