AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

for specific analytical need. In Herbalife Nutrition, NMR is applied for identification of botanical materials and quantitation of chemical markers in those materials. It is used for identification tests of Aloe vera raw materials and finished products in both powder and liquid forms. It is also used for characterization and differentiation of similar tea extract materials. Their simi- larities and differences could be determined by NMR together with statistical analysis. Another example of NMR application is quantitation of rebaudiosides in stevia materials is used as an example. In addition, NMR is also being evaluated for deter- mination of other chemical constituents of food and dietary supplement products. Presenter: Congmei Cao, Herbalife Nutrition, Torrance, CA, USA, Email: congmeica@herbalife.com P-M-008 Maria Monagas , Joshua Bhattacharya , Jennifer Fedorowski , Gabriel Giancaspro , U.S. Pharmacopeia (USP), Rockville, MD, USA; Débora Frommenwiler , Eike Reich , CAMAG Scientific, Inc., Muttenz, Switzerland Revision of the USP Cranberry Liquid Preparation Monograph Cranberry fruit is the largest individual botanical ingredient used in dietary supplements in the U.S. Cranberry dietary supplements are mainly derived from the juice manufacturing process. Both the juice and remaining material after juice pressing (pomace) can be further processed into the following ingredients: juice concentrate, dry juice, and juice or pomace extracts. Cranberry juice corresponds to specifications described in the current USP Cranberry Liquid Preparation monograph and is the only existing pharmacopeial standard for Cranberry ( USP 42 ) and a reference for other pharmacopeias worldwide. USP has been actively working on development and improvement of analytical methods for revision and creation of new standards for cran- berry ingredients and finished products. This abstract summarizes work carried out for the revision of the USP Cranberry Liquid Preparation monograph. The following revisions will be proposed in the Pharmacopeial Forum (PF) 45(6): Change in Title from Cranberry Liquid Preparation to Cranberry Juice , change in the Definition to include the requirements of refractive index and corresponding percentage (%) total solids, inclu- sion of the new test Identification B, HPTLC for Articles of Botanical Origin < 203 >, inclusion of the new test Identification C, HPLC Profile of Flavonoids, new proposal for the test for Composition, HPLC Content of Fructose and Dextrose, inclusion of the new Specific test, Limit of Benzoic acid , and inclusion of Contaminants . Presenter: Maria Monagas, U.S. Pharmacopeia (USP), Rockville, MD, USA, Email: mjm@usp.org P-M-009 Joseph O’Donnell , DSM Nutritional Products, Belvidere, NJ, USA Single Extract Determination of Vitamins A, D3, E, and Beta-Carotene Using UPC2 Technology The analytical determination of multiple analytes from a single sample has long required numerous instruments as well as

multiple extractions. UPC 2 allows for the quantitative determina- tion of multiple analytes from a single sample extract. Fat soluble vitamins play critical roles in our wellness including heart health, bone maintenance, eye and vision health, and even an essential role in blood clotting. For this application, a Waters ACQUITY UPC 2 (UltraPerformance Convergence Chromatography) PDA system was used to analyze and quantitate fat soluble vitamins A, D 3 , and E, as well as Beta-Carotene from a single premix sample extract. UPC 2 uses cheap, non-toxic compressed CO 2 as the primary mobile phase to provide exceptional selectivity and resolving power. Because of this ability to vary mobile phase strength, temperature, and pressure, scientists can exhibit better control of retention and separation that are often a challenge to separate by many other techniques. For comparison, all 4 components can be fully analyzed from a single injection in just 12 minutes on UPC 2 , versus a total time of 60 min using 4 sepa- rate HPLCs. In this application, the linearity of the method was determined. Quantitation was done using sample peak areas calculated against single point standard averages. Content uniformity tests were performed to determine the precision of each analyte, as well as recovery studies. The benefits of UPC 2 technology and all method data will be presented. Presenter: Joseph O’Donnell, DSM Nutritional Products, Belvidere, NJ, USA, Email: joseph.odonnell@dsm.com P-M-010 Tiffany Gallegos-Peretz , Boris Nemzer , FutureCeuticals, Momence, IL, USA A Method for the Quantitative Analysis of Total Curcuminoids in Turmeric and Nutritional Supplements Containing Turmeric by HPLC-DAD The food industry’s focus to natural food colorants has increased significantly due to safety concerns of artificial colorants. Turmeric contains curcumin, the main pigment present in the rhizomes of Cucuma longa L., along with other cucuminoids. This study validated a test method for the quantitative analysis of total curcuminoids in turmeric powders, extracts and nutritional supplements containing turmeric developed at FutureCeuticals (Momence, IL). This method is capable of quantitating three curcuminoids (curcumin, demethoxycurcumin, and bisde- methoxycurcumin) as well as tetrahydrocurcumin, the major metabolite from the reduction of Curcumin. Turmeric powder and extract samples used in the validation were commercially sourced by FutureCeuticals. The dietary supplements used in the method validation, Spectra™ and S7™, were manufac- tured by FutureCeuticals. Curcuminoids were extracted from samples using a 50% acetonitrile:water solution and analyzed using High Performance Liquid Chromatography Diode Array Detector (HPLC-DAD) with detection at 280 and 425 nm. The method validation established individual Curcuminoid detection limits from 0.02 to 1.6%. The validation incorporated method precision, accuracy, ruggedness, and robustness to demon- strate the method is fit-for-purpose. The method holds potential for future analysis of the bioavailability of dietary supplements containing turmeric. Presenter: Tiffany Gallegos-Peretz, FutureCeuticals, Momence, IL, USA, Email: tiffany.gallegosperetz@futureceuticals.com

36 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL