AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

P-M-019 Larry Zeng , Yolanda Xiong , Leo Li , Herbalife NatSource (Hunan) Natural Products Co., Ltd, Changsha, China; Congmei Cao , Yanjun Zhang , Peter Chang , Gary Swanson , Herbalife Nutrition, Torrance, CA, USA A Single Laboratory Validation of a Test Method for the Quantitation of Punicalagins A&B and Ellagic Acid in Pomegranate ( Punica granatum ) Fruit Rind, Fruit Rind Powder, and Fruit Rind Extract by HPLC Pomegranate ( Punica granatum ), an edible fruit originated in the Middle East, has been used as a traditional medicine for the treatment of pain and inflammatory conditions such as peptic ulcers. Punicalagins A&B and ellagic acid are the most important bioactive chemical components in pomegranate ( P. granatum ) fruit rind. Accurate quantitation of these components is critical for quality control. A simple and quick HPLC method was developed for the quantitation of punicalagins A&B and ellagic acid in pomegranate fruit rind, fruit rind powder and fruit rind extract. The pomegranate materials were extracted with DMSO by sonication and diluted with water, then the amount of punicalagins A&B and ellagic acid were determined by HPLC. This study revealed that 60-80% DMSO in water can achieve not only great extraction efficiency but also exclude the interference of punicalagins A&B. Its precision, accuracy, linearity, specificity and ruggedness were validated following AOAC Guidance for Single Laboratory Validation Procedure. The recoveries of punicalagins A&B and ellagic acid from pomegranate fruit rind, fruit rind powder and fruit rind extract are 84-104%. The method is reliable for the quantitation of punicalagins A&B and ellagic acid in pomegran- ate fruit rind, fruit rind powder and fruit rind extract. It is suitable for the quick analysis for quality control purposes. Presenter: Yanjun Zhang, Herbalife Nutrition, Torrance, CA, USA, Email: yanjunzh@herbalife.com

with only four minutes of chemist time required per sample for the extraction procedure. Presenter: Daniel Hengst, Eurofins Food Integrity and Innovation, Madison, WI, USA, Email: DanielHengst@eurofinsUS.com P-M-017 Dana Krueger , Krueger Food Laboratories, Inc., Chelmsford, MA, USA Analysis of Ginger Flavor Compounds in Carbonated Soft Drinks A method for the determination of the ginger flavor compounds 6-gingerol, 6-shogaol and 8-gingerol in carbonated soft drinks is presented. Degassed samples are extracted with hexane/ ethyl acetate. The extract is dried, evaporated, internal standard added and taken up in 50% acetonitrile for HPLC analysis. HPLC is performed using a C18 reversed phase column, an acetoni- trile/water solvent gradient and UV detection at 280 and 230 nm. Ginger flavor compounds at concentrations typically found in ginger ale and similar ginger flavored soft drink beverages are determined with high recovery. Presenter: Dana Krueger, Krueger Food Laboratories, Inc., Chelmsford, MA, USA, Email: dkrueger@kfl.com P-M-018 Isaac Lee , Quanyin Gao , Herbalife Manufacturing, LLC, Lake Forest, CA, USA; Peter Chang , Gary Swanson , Herbalife Nutrition, Torrance, CA, USA Rapid Quantitation of Total Fat in Sunflower Oil Powder Using Time-Domain Nuclear Magnetic Resonance (TD-NMR) Spectroscopy A rapid TD-NMR method was developed to quantitate total fat in high oleic sunflower oil powder. Currently, industry has two methods for fat quantitation:gravimetric analysis after ether extraction (AOAC 933.05 and AOAC 989.05), and GC-FID (AOAC 996.06). The gravimetric method takes a day using very flammable solvents. The GC-FID method takes two days using harsh chemicals for hydrolyzation and derivatization. In contrast, TD-NMR method takes only an hour and the sample is analyzed “as is.” Hahn-echo pulse program was used with two sequential radio frequencies to generate a spin echo followed by intensity measurement. An echo time of 7 ms and a recycle delay (RD) time of 1.5 sec were employed through- out the experiment. Five concentrations of sunflower seed oil standards resulted in a calibration curve with an R 2 value of 0.99997. Using this calibration curve, multiple lots of high oleic sunflower oil samples were analyzed for fat amount, and the results were compared to the traditional gravimetric method post ether extraction. Using TD-NMR, an average of 47% total fat was obtained, and an average of 46% total fat was acquired for gravimetric method. Individual total fat result obtained by TD-NMR was ±1% of ether extraction gravimetric method. The TD-NMR method is not only a fast and accurate sample analysis technique, but it is also friendly to the envi- ronment without chemical/reagent waste disposal and safer without flammable solvent usage. Presenter: Isaac Lee, Herbalife Manufacturing, LLC, Lake Forest, CA, USA, Email: isaacle@herbalife.com

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