AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

P-M-020 Shawn Yang , Herbalife NatSource (Hunan) Natural Products Co., Ltd, Changsha, CA, USA; Luke He , Suki Chen , Leo Li , Herbalife NatSource (Hunan) Natural Products Co., Ltd, Changsha, China; Zheng Quan , Silva Babajanian , Peter Chang , Gary Swanson , Zhengfei Lu , Herbalife Nutrition, Torrance, CA, USA Integration of DNA-Based Molecular Analysis as an Orthogonal Analytical Approach in Industry Botanical Identification Botanical materials and ingredients present challenges in iden- tification due to their profile diversity. In many cases, multiple orthogonal tests using essentially different methodologies are required to obtain reliable botanical identification results. Among those tests, DNA-based molecular analysis becomes an ideal approach to complement widely accepted morphological and chemical methods. Herbalife Nutrition has added DNA-based molecular analysis in its botanical identification practice and gained experiences. In this study, we present data and examples collected from routine testing and show the low variations of DNA barcode sequences compared to the fluctuations of chem- ical profiles observed in industry botanical materials. Secondly, although DNA and chemical methods identify botanical mate- rials from different perspectives, there are strong correlations between results from DNA-based methods and compendium chemical analytical methods, e.g., results from DNA barcoding and HPLC in Panax ginseng identification. Finally, for botani- cals with limited reference materials and testing experiences, DNA-based molecular analysis can reduce the risks of misiden- tification with high efficiency and low cost. In summary, these data and examples demonstrate that integrating DNA-based molecular analysis to provide parallel characterization in botan- ical identification is essential to advance the quality control of botanical products. Presenter: Zhengfei Lu, Herbalife Nutrition, Torrance, CA, USA, Email: zhengfeil@herbalife.com P-M-021 Karen Andrews , Pavel Gusev , Sushma Savarala , Phuong-Tan Tey , Laura Oh , Ronelle Bautista , Pamela Pehrsson , U.S. Department of Agriculture, Beltsville, MD, USA; Johanna Dwyer , Adam Kuszak , Rebecca Costello , Leila Saldanha , National Institutes of Health, Bethesda, MD, USA Analytical Content and Methods of Analysis for Vitamins and Minerals in Prescription Prenatal Multivitamin/ Mineral Products Most pregnant women take prenatal multivitamins (PMVM). Dietary surveys use only labeled nutrient amounts, which may lead to inaccurate estimates of nutrient adequacy. We inves- tigated the relationship between the labeled and analytically measured content of nutrients in a representative sample of prescription (Rx) PMVM. Multiple lots of 24 products (61% of the market in 2015/16) were tested for their vitamin and mineral content. Laboratories were selected based on quality control

results, including National Institute of Standards and Technology Standard Reference Materials. All but 3 nutrients (vitamins E, B-12, iodine) were tested in multiple laboratories to double- check unusual levels or highly variable results among lots. Most vitamins were measured by high performance liquid chroma- tography with mass spectrometry, ultraviolet/visible and/or photodiode array detectors. Most minerals were measured using inductively coupled plasma spectroscopy. Sample preparation, extraction and detection protocols were modified and optimized, especially for softgel products. Analyses showed mean overages of ≥ 20% for 6 vitamins (folic acid, niacin, riboflavin, vitamins A, B-12, D); 10-15% for vitamins B-6, C, E; and slightly below (-4%) label results for thiamin. Analytical content was close to labeled content for minerals (~1-11% above label). Therefore, nutrient intakes from Rx PMVM, especially for vitamins, could be underestimated if only using label information. Presenter: Karen Andrews, U.S. Department of Agriculture, Beltsville, MD, USA, Email: karen.andrews@usda.gov P-M-022 Maria Monagas , Amanda Guiraldelli , Rafael Maranho , Nam-Cheol Kim , Gabriel Giancaspro , U.S. Pharmacopeia (USP), Rockville, MD, USA Anthraquinones and Glycosylated Dianthrone Sennosides ( C. senna, C. angustifolia or C. acutifolia ) is a herbal medicine containing glucoside compounds found in several commercial laxative products and FDA-approved nonprescrip- tion drugs. The main constituents of Sennosides are glycosidic dianthrones such as sennosides A and B, both di- O -gluco- sides of rhein dianthrones, and sennosides C and D, which are di- O -glucosides of rhein-emodin dianthrones. A simple analyt- ical method by LC-UV was developed for anthraquinones and glycosylated dianthrones quantitation in Sennosides, and to characterize the material by LC-UV-ESI-QToF. In total, 46 compounds were identified in the extract, being the two major compounds identified as sennosides A (31.8%) and B (39.3%). Sennoside G was able to be well-resolved from its diastereo- mer sennoside A represented 1% of the extract. Diglycosylated heterodianthrones were also detected being the two major compounds identified as sennoside C (2.8%) and D (1.9%). Several triglycosylated homodianthrones were identified and detected in relevant amounts (3.9%), as well as monoglyco- sylated (5.4%) and diglycosylated dianthrones (1.7%). The precision, linearity and LOQ for the proposed new method were evaluated and met USP validation requirements for Sennosides A, B, C, D, and Rhein. The proposed new method shows to be a good alternative in the quality control assay of the glycosylated anthraquinones and aglycons in sennosides extract by LC-UV. Presenter: Maria Monagas, U.S. Pharmacopeia (USP), Rockville, MD, USA, Email: mjm@usp.org Characterization by LC-UV-ESI-QToF and Quantification by LC-UV in Sennosides

40 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL