AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

negative binomial distribution, an R program was developed to calculate an operating characteristic curve for a specific gluten sampling plan design. The effect of change in sampling plan parameters on the risks associated with gluten sampling plan designs was evaluated. Increasing sample or test portion size resulted in reducing the probability of accepting a lot containing >20 ppm gluten. For example, at a lot gluten concentration of 25 ppm, an increase in sample size from 0.5 to 5 kg for a 20 g test portion reduced the buyer’s risk from 42.79 to 24.18%. Increasing the number of laboratory samples or decreasing the accept/reject limit also reduced the buyer’s risk but increased the seller’s risk. The developed R program may help regulators and industry evaluate sampling plans to reduce the risk levels. Presenter: Girdhari Sharma, U.S. Food and Drug Administration, Laurel, MD, USA, Email: Girdhari.Sharma@fda.hhs.gov P-M-052 Daniel Hengst , Chad Scheuerell , Hannah Poe , Eurofins, Madison, WI, USA Menadione is a synthetic form of Vitamin K used to supple- ment animal feeds. The K vitamins, which include Menadione (Vitamin K3), Phylloquinone (Vitamin K1), and Menaquinone (Vitamin K2), have numerous physiological roles, with the primary role being a cofactor in the coagulation of blood. Accurate analysis of Menadione in feed premix is essential, since a deficiency of this vitamin will result in adverse health effects in the animal, yet an overabundance of the compound can be toxic. A method was developed and validated utilizing Supercritical Fluid Chromatography with Diode Array or Tandem Mass Spectrometer detection for the resolution and analysis of Menadione in animal feed premixes. This method has a rapid extraction procedure requiring minimal analyst labor and quick chromatography, with Menadione resolved in less than 3 minutes. Presenter: Daniel Hengst, Eurofins, Madison, WI, USA, Email: DanielHengst@EurofinsUS.com P-M-053 Yin Ling Chew , Jie Xing , Zhaoqi Zhan , Shimadzu (Asia Pacific) Pte. Ltd, Cintech, Singapore; Eleanor Hor , School of Physical and Mathematical Sciences, Nanyang Technological University, Cintech, Singapore; Lee Qi Zong , Noah Luzheng , National University of Singapore, Department of Chemistry, Cintech, Singapore; Muhammad Averus , Suwiton Suwiton , Martin Uli Tua , PT Saraswanti Info Genotech, Bogor City, Indonesia; Robert Clifford , Shimadzu Scientific Instruments, Inc., Columbia, MD, USA LC-MS/MS Method for Sensitive Detection and Quantitation of 8 Water-Soluble Vitamins in Infant Milk Powder Water-soluble B vitamins are essential nutrients needed by human body to function properly. Since B vitamins are not produced in adequate amounts in human body, it is important to obtain them from food sources. Low levels of B vitamins in breastfeeding mothers may cause their babies slow growth. Therefore, infant formulas must include proper amounts of nutri- ents including B vitamins. The Dietary Reference Intake and the The Analysis of Menadione in Feed Premixes Using Supercritical Fluid Chromatography

Food Additive have issued the reference value of infants’ daily requirement of B vitamins in infant formula. HPLC and LC-MS/ MS methods have used in analysis of B vitamins and additives, either limited to few compounds in HPLC method or required time-consuming sample clean-up method due to the complicated matrix of infant formula. Here, we present a LC-MS/MS method for simultaneous determination of 8 water-soluble B vitamins in infant formula with 5 isotope labelled internal standards, with a simple sample extraction procedure Presenter: Yin Ling Chew, Shimadzu (Asia Pacific) Pte. Ltd, Cintech, Singapore, Email: chewyinling@shimadzu.com.sg P-M-054 Deepali Mohindra , Thermo Fisher Scientific, Sunnyvale, CA, USA; Sylvia Grosse , Mauro de Pra , Frank Steiner , Thermo Fisher Scientific, Germering, Germany Simultaneous Determination of Water- and Fat- Soluble Vitamins in Tablets and Energy Drinks by Using a Novel Vanquish Flex Duo UHPLC System Vitamins can be classified as water-soluble vitamins (WSV) or fat-soluble vitamins (FSV). The differing hydrophobicity of these compounds means that simultaneous determination via liquid chromatography typically requires complex hardware and software solutions. In this work, we introduce an effective workflow that overcomes these challenges based on a novel Vanquish Flex Duo system for Dual LC. This system enables the independent and simultaneous use of two independent columns and methods to optimize for FSV and WSV. Compared to other solutions, this approach is simple to implement, allows the use of more optimized methods, and increases throughput thanks to the simultaneous use of two columns with two methods and faster analysis cycles. Moreover, potential issues such as incompatibil- ity of the mobile phases in the shared flow path, such as buffer salt precipitation in high-organic solvent, are eliminated. In this instance, the Acclaim PolarAdvantage II (PA2) column is used for both vitamin classes. The PA2 column features a polar-em- bedded stationary phase that effectively operates over a wide range of mobile phase conditions and is highly suitable for the separation of components with very diverse hydrophobicity, such as vitamins. Presenter: Deepali Mohindra, Thermo Fisher Scientific, Sunnyvale, CA, USA, Email: deepali.mohindra@thermofisher.com P-M-055 Chung Cho , Katherine Ivens , Eric Garber , U.S. Food and Drug Administration, College Park, MD, USA; William Nowatzke , Kerry Oliver , Radix BioSolutions, Georgetown, TX, USA; Jason Robotham , BioFront Technologies, Tallahassee, FL, USA; Mansour Samadpour , IEH Laboratories and Consulting Group Inc., Lake Forest Park, WA, USA; Thomas Grace , Bia Diagnostics LLC, Colchester, VT, USA Method Extension of xMAP ® Food Allergen Detection Assay for Sesame The Food Allergen Labeling and Consumer Protection Act of 2004 (FALCPA) imposes strict requirements for labeling of foods containing one of the eight major food allergens. Recently, there has been evidence that sesame allergies may be a growing

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