AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

concern in the U.S. About 0.1-0.2% of the North Americans are allergic to sesame and anaphylactic reactions have been reported. Detecting and quantifying sesame in various food samples is critical to the health and safety of allergic populations by ensuring the accuracy of food labeling. The xMAP ® Food Allergen Detection Assay (xMAP ® FADA) has been validated for regulatory use and has an advantageous modular plug- and-play design that allows the inclusion of additional analyte targets. By using commercially established antibodies, through method extension, three sandwich binding assays were config- ured for the detection of sesame and incorporated into the Buffered-Detergent Protocol of the xMAP ® FADA. The xMAP ® FADA successfully detected and confirmed the presence of sesame in muffins, spice mix, canola oil, and sesame oil with a limit-of-detection (LoD) less than 1 ppm. Cross-reactivity with cashew antibodies was easily distinguished based on ratio anal- ysis of the complimentary bead sets. As both raw and toasted sesame were successfully detected by the assay, the validated xMAP ® FADA was expanded successfully to include sesame. Presenter: Chung Cho, U.S. Food and Drug Administration, College Park, MD, USA, Email: Chung.Cho@fda.hhs.gov P-M-056 Tairo Ogura , Toshiya Matsubara , Ichiro Hirano , Shimadzu Japan, Kyoto, Japan; Yuka Fujito , Alan Owens , Shimadzu USA, Columbia, MD, USA Simultaneous Analysis of Multiple Food Allergen and its Detection from Processed Food Food allergy becomes a public health concern and its prev- alence is currently estimated up to 10%. The Food Allergen Labeling and Consumer Protection Act (FALCPA) requires that food manufacturers label food products intentionally containing food ingredients such as milk, eggs, fish, crustacean shellfish, tree nuts, peanuts, wheat, and soybeans. These have been identified as the eight major food allergens. While ELISA is frequently used technique to detect allergenic proteins, liquid chromatograph tandem mass spectrometry (LC-MS/MS) becomes an alternative technique in terms of high selectivity, sensitivity, and capability to analyze multiple allergens simultaneously. It is also expected that LC-MS/MS technique can overcome existing issue of ELISA such as false detection of allergens in processed foods. Presenter: Alan Owens, Shimadzu USA, Columbia, MD, USA, Email: amowens@shimadzu.com P-M-057 Yusuke Takemori , Yui Higashi , Takero Sakai , Shimadzu Japan, Kyoto, Japan; Ryo Takechi , Alan Owens , Shimadzu USA, Columbia, MD, USA; Motoki Sasaki , Narihiro Suzuki , Ise Kadoya Brewery, Ise, Japan Classification and Visualization of Beer Quality Using GC-MS and GC-FID Despite the same brand and identical brewing techniques, it is widely known that beer taste and quality is not consistent from plant to plant. Therefore, brewers normally check beer quality mainly by sensory test method, and in turn, adjust beer taste in order to reduce these differences as much as possible. In this poster, by metabolomics profiling using GC-MS and GC-FID, we introduce a new approach towards classification and

visualization of beer quality to identify how specific components influence taste from plant to plant. Presenter: Alan Owens, Shimadzu USA, Columbia, MD, USA, Email: amowens@shimadzu.com P-M-058 Mandy Sperry , Dave Almy , Driksna Dana , Neogen Corp., Lansing, MI, USA Validation of Reveal 3D ® Coconut Test Kit Neogen Corporation has developed a highly sensitive lateral flow assay for the detection of coconut protein on surfaces, clean in place rinses, and liquid foods. The test is capable of detecting 1 ppm coconut protein in a variety of matrices. A spike recov- ery study with soy milk and wheat flour as the sample showed partial-recovery at 0.2 and 0.4 ppm and 100% detection at 0.6 ppm and greater in both commodities. Surface swab recovery testing demonstrated 100% detection of 1 ug/100 cm 2 coconut protein on stainless steel, plastic, and nonstick surface. Partial recovery was observed at 0.5 ug/100 cm 2 coconut protein on plastic and nonstick surfaces. Coconut protein spiked into a variety of working strength cleaners common to the food and beverage industry was detected at 1 ppm and above. Market basket screening demonstrated coconut detection in a variety of processed foods. Cross-reactivity testing showed no cross-reac- tivity in several non-coconut foods samples. Ruggedness testing (multi-user, multi-day, multi-lot) showed consistent results for several levels of spiked samples. Presenter: Mandy Sperry, Neogen Corp., Lansing, MI, USA, Email: msperry@neogen.com P-M-059 Mandy Sperry , Driksna Dana , Dave Almy , Neogen Corp., Lansing, MI, USA Validation of Veratox ® Coconut ELISA Test Kit Neogen Corp. has developed a highly sensitive, fully quantita- tive assay for the detection of coconut protein on surfaces, clean in place rinses and food products such as beverages, cookies, crackers, chocolate bars, ice cream, and cereals. The kit offers a quantitation range of 1-25 ppm coconut protein that can be extended by dilution of positive extracts. The kit development focused on sensitivity, the ability to accurately detect processed samples, such as UHT beverages, and superior cross-reactivity. Detection limits, cross reactivity, ruggedness, robustness, and a Beta site evaluation were completed during the validation of the assay. The LOD was found to be 0.53 ppm in cookie mix and 0.07 ppm in soy milk. The LOQ was found to be 1.41 ppm in cookie mix and 0.33 ppm in soy milk. Slight cross reactivity (≤0.0002%) was observed for cherry pits, peach pits, hazelnuts, and pumpkin or sunflower seeds. A screen of several store- bought foods demonstrated that the kit is capable of detecting coconut protein in a wide variety of commodities, even highly processed foods. A multi-parameter validation study showed consistent recovery for a range of spiked commodities. Presenter: Mandy Sperry, Neogen Corp., Lansing, MI, USA, Email: msperry@neogen.com

50 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL