AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

proteomic analysis by targeted high-resolution mass spectrom- etry or multiple-reaction monitoring by triple-quadrupole mass spectrometry with stable isotope-labelled internal standards, using specific marker peptides of major gluten proteins. Protein extracts were also analysed by immunoblotting with IgE (0.7 to > 100 kU/L) of patients with confirmed wheat allergy, or with commercial anti-gliadin IgG. First results of both methods showed the presence of marginal amounts of gluten peptides in the salmon samples, indicating that carry-over from feed to fish might occur to a very limited degree. Presenter: Christiane Fæste, Norwegian Veterinary Institute, Oslo, Norway, Email: christiane.faste@vetinst.no P-M-079 Sean Austin , Societe des produits Nestlé SA, Lausanne, Switzerland Determination of Sugars by Anion Exchange Chromatography Using a Dual Eluent Generator High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) is a powerful tool for the analysis of mono- and oligosaccharides. It has the advan- tage of high sensitivity and excellent resolving power without the need for derivatisation of the analytes. Perhaps one of the most time-consuming tasks of setting up the system is prepa- ration of the mobile phase, requiring extensive degassing to eliminate carbonate. Last year a dual eluent generator (Dual EG) was introduced, able to generate gradients of both KOH and potassium methane sulfonate (KMSA) needing only water to be prepared for the mobile phase. One of the most common analyses required in the food industry is the analysis of sugars (glucose, galactose, fructose, sucrose, lactose and maltose). The separation and quantitative determination of these six sugars is easily performed on a CarboPac PA20 (7 µm, 3 x 150 mm) using a gradient of sodium acetate and NaOH. The same analysis can be performed on a prototype CarboPac PA20 (4 µm, 1 x 150 mm) using a gradient of KMSA and KOH. We also observed that while it is necessary to use post-column addition of NaOH when running the analysis on a standard system, it was not necessary on the system using the 1 mm column. The system was also applied for the analysis of fructans in infant formula according to AOAC 2016.14, and results were comparable to the standard method. Presenter: Sean Austin, Societe des produits Nestlé SA, Lausanne, Switzerland, Email: sean.austin@rdls.nestle.com P-M-080 Thierry Bénet , Antoine Lévêques , Denis Cuany , Sean Austin , Societe des Produits Nestlé SA, Lausanne, Switzerland; Begoña Casado , Societe des Produits Nestlé SA, La Tour-de-Pleiz, Switzerland Determination of Human Milk Oligosaccharides in Infant Formula Human milk oligosaccharides (HMO) are a major component of human milk, and may be responsible for some of the benefits experienced by breast-fed infants. Recent technical develop- ments have meant that some HMO can now be manufactured at industrial scale, and are available for addition to infant formula. We developed a method for the analysis of oligosaccharides in

human milk, covering around 20 of the major HMO. The method was based on labeling the oligosaccharides with a fluorescent tag, 2-aminobenzamide (2AB), and separation of the HMO by UHPLC using a HILIC stationary phase. With little adaptation, the same method can also be applied for the analysis of HMO in infant formula. Single-laboratory validation of the method for the determination of 2’FL and LNnT in formula demonstrated the method was able to achieve trueness (based on spike-recover- ies) of 94–104% and intermediate precision [RSD(iR)] between 2 to 7.5%. The method can easily be expanded to incorporate other HMO, as already demonstrated in human milk. Presenter: Sean Austin, Societe des Produits Nestlé SA, Lausanne, Switzerland, Email: sean.austin@rdls.nestle.com P-M-081 April Schumacher , Gabriela Lopez , Sarah Sykora , 3M, St. Paul, MN, USA Performance Validation of a Rapid Method to Detect Coconut Proteins in Environmental Samples, Food Ingredients, and Processed Food Products The 3M™ Coconut Protein Rapid Kit utilizes lateral flow devices (LFD) that are immunochromatographic test method manu- factured with antibodies designed for the specific detection of coconut proteins. This method is intended for screening of clean-in-place (CIP) final rinse water, environmental swabs, food ingredients and processed food products for the pres- ence of specific allergenic proteins. The objective of this study was to validate the performance of a rapid immunochromato- graphic method according to AOAC appendix H ( J. AOAC Int. 2012) to specifically detect coconut proteins in foods. The method was assessed for cross reactivity with 21 different food commodities including raw and roasted nuts, seeds, grains, legumes, and other foods. Food products were first screened for the absence of coconut and then spiked with 10 ppm of coco- nut protein. Probability of detection curves were constructed utilizing incurred cookies, blueberry yogurt, soy milk, chocolate powder, vanilla ice-cream, CIP water and environmental swab. Lot consistency and robustness were also assessed. The 3M TM Coconut Protein Rapid Kit is robust requiring minimum sample preparation and short incubation periods. Cross reactivity was not determined in any of the commodities evaluated in the study. All data collected indicate that the 3M TM Coconut Protein Rapid Kit can detect coconut proteins at concentrations as low as 2 ppm. The evaluated method was granted AOAC Performance Tested Method SM certification number 061903. Presenter: April Schumacher, 3M, St. Paul, MN, USA, Email: ajschumacher@mmm.com P-M-082 Despoina Lyda Voulgari , Konstantina Badra , Nikolaos Natsaridis , Fotini Dimakou , Georgios Papageorgiou , Antonios Ntantasios , Sotiria Drakouli , Sotirios Athanasiou , R&D Department, Prognosis Biotech S.A., Larissa, Greece Validation of the First Lateral Flow Test for the Quantification of Histamine in Seafood Samples High levels of Histamine (HA) may cause scombroid poisoning in humans and therefore the levels of HA should not exceed 50

56 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL