AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Monday

P-M-084 Ben Pointer , Ngoctran Cao , Eurofins, Des Moines, IA, USA Rapid and Simultaneous Analysis of Thiamine and Riboflavin in Infant Formula by Ultra-Performance Liquid Chromatography and Post-Column Derivitization Thiamine (Vitamin B1) and Riboflavin (Vitamin B2) are water-sol- uble vitamins of the B complex that play an important role in human health, and are added to infant and adult nutritional formulas. There are several different official methods used to quantitate these vitamins, most commonly used in analytical laboratories are the fluorimetric methods AOAC 986.27 (for thiamine as thiochrome) and AOAC 970.65 (for riboflavin), as well as a liquid chromatography method, EN 14152:2003 for the determination of riboflavin. Eurofins Nutrition Analysis Center has developed a method based on EN 14152:2003 and AOAC 986.27 for the simultaneous determination of thiamine and riboflavin. In this method, samples are acid hydrolyzed, then treated with phosphatase to cleave thiamine phosphate derivatives into free thiamine. Analysis is then performed by reverse-phase ultra-performance liquid chromatography with tandem fluorimetric detectors. Riboflavin content is determined in the first detector, and then thiamine is converted to the fluores- cent derivative thiochrome inside of a reaction coil, which is then detected in the second detector. The single-laboratory validation (SLV) of this method is discussed. Presenter: Ben Pointer, Eurofins, Des Moines, IA, USA, Email: BenPointer@EurofinsUS.com Heather Hawk , Hongjian Ding , U.S. Food and Drug Administration, Jefferson, AR, USA; Monica Pava-Ripoll , U.S. Food and Drug Administration, College Park, MD, USA; Randy Self , U.S. Food and Drug Administration, Bothell, WA, USA Filth and Decomposition in Foods: Overview and Selected Research The Food and Drug Administration (FDA) works to protect consumers from foods which may contain filth. Filth includes contaminants such as rat, mouse, and other animal hairs and excreta; whole insects, insect parts, and excreta; and other extra- neous materials which, because of their repulsiveness, would not knowingly be eaten or used. The FDA also works to protect consumers from decomposed seafood. Because the presence of decomposition in seafood can be indicative of undesirable exposures associated with lax harvesting, handling, manufactur- ing, storage, and/or distribution practices that are conducive to microbial contamination, the reliable detection of decomposition provides an efficient and effective screening of food products that may otherwise contain contaminants that could poten- tially pose a hazard to consumers’ health. Selected research in the areas of filth and decomposition in foods: 1. Detection of Foodborne Bacterial Pathogens from Individual Filth Flies 2. MitochonTrakr: a reference collection of high quality mitochon- drial genomes for detecting insect species in food products 3. Comparing SVM and ANN based Machine Learning Methods for Species Identification of Food Contaminating Beetles 4. How MISCELLANEOUS P-M-085

ppm in seafood, (EU limit). So far, there is not an established Lateral Flow (LF) method for the HA quantification because of the limited LF technology. ELISA is one of the most reliable methods and usually lasts 20-60 min. The aim of this study was the valida- tion of a quantitative 5-minute LF assay by the determination of HA recovery in spiked fish samples and Reference Materials (RMs) as well as the comparison with ELISA results. The quantification of HA was conducted with LF Symmetric Histamine (S9048, S9048004) and ELISA Bio-Shield Histamine (B6096, B6096007) (Prognosis Biotech S.A.). Both methods do not use the acylation step and last 5 and 20 min, respectively. Sample preparation consists of extraction and dilution normal- ization. Fresh and thawed frozen raw HA-free fish samples (anchovy, sardine, tuna, cod, mackerel, fish meal) were spiked (50 and 100 ppm). The recovery of FAPAS RMs was also deter- mined. The LF method’s recovery and CV% of all spikes and RMs were within an acceptable range and almost identical to ELISA method levels. Additionally, there was no significant difference in the results between fresh and thawed frozen samples. This innovative Symmetric LF kit provides results comparable to those of ELISA, reducing considerably the analysis time. Thus, Symmetric Histamine is the first specific LF product to quantify HA in seafood. Presenter: Georgios Papageorgiou, R&D Department, Prognosis Biotech S.A., Larissa, Greece, Email: g.papageorgiou@prognosis-biotech.com P-M-083 Derrell Johnson , Uma Sreenivasan , MilliporeSigma, Round Rock, TX, USA; Judy Cao , Kevin Ray , MilliporeSigma, St. Louis, MO, USA Evaluation of a Surrogate Matrix for Whey Protein Quantitation in Infant Formula by LC-MS Infant formula, an alternative to breast milk, provides nutrition for the growth of babies. For example, α -lactalbumin (LA), a predominant whey protein in infant formula, plays important roles in several biological processes including lactose biosynthe- sis and immune system regulation. On the other hand, another whey protein, β -lactoglobulin (LG), present in bovine milk but absent in human milk, has been reported as one of the allergens in infant formula. Therefore, it is essential to develop quality control methods for the quantitation of LA and LG in infant formu- las. Among various techniques which have been developed for the analysis of whey proteins, liquid chromatography-mass spec- trometry (LC-MS) is becoming more widely adopted. However, one of the challenges is the selection of surrogate matrix for the preparation of calibration standards. In this poster, we will present our approach to determine the suitability of a surrogate matrix for the quantitation of LA and LG in infant formula by LC-MS. Presenter: Derrell Johnson, MilliporeSigma, Round Rock, TX, USA, Email: derrell.johnson@milliporesigma.com

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