AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

in the sample. Validation of the method indicated an ability to reliably detect A1 content down to 2% of total beta-casein in various milk powder samples. Presenter: Jeffrey Shippar, Eurofins, Madison, WI, USA, Email: jeffreyshippar@eurofinsus.com P-T-008 Deepali Mohindra , Thermo Fisher Scientific, Sunnyvale, CA, USA; Suresh Murugesan , Dasharath Oulkar , Thermo Fisher Scientific, New Delhi, India Trace Level Quantitation of Trace Metals in Milk and Milk Products by Using ICP-MS Multi-elemental quantification method of trace elements in milk and milk products using microwave assisted closed vessel acid digestion followed by ICP-MS (Thermo Fisher Scientific iCAP-RQ) analysis. The milk, cheese, and butter samples are digested with tri-acid combination (Nitric acid, Hydrogen peroxide and Hydrochloric acid) by addition of 20 ug/L, internal standards (Sc, Ge, Y, In, Tb, Rh, Tl, Ir, and Bi) with concentration of 20 ug/L. Gold solution (200 µg/L) is used as stabilization reagent in sample preparation to stabilize Mercury and to reduce memory effect. Also, the microwave digestion was slightly get extended ramping (30-40 min) and hold time (20-30 min) to get complete digestion reflected in excellent recoveries. The prepared samples are quantified against the neat standard calibration curve plotted with 6 points. The optimized method was validated for 20 elements (Al, As, Be, B, Ba, Cd, Co, Cu, Cr, Fe, Hg, Li, Mn, Mo, Ni, Pb, Sb, Sn, V, and Zn). The method performance checked for linearity, sensitivity, selectivity, repeat- ability, accuracy at 3 different levels in accordance with AOAC 2015.01 guideline. The R 2 values are greater than 0.995 for all target elements. The limit of quantitation (LOQs) are verified through the spiked samples and evaluated for % recovery and % RSD. Excellent average recoveries achieved (81-112%) for all the spiked levels in compliance with AOAC 2015.01 guideline. This method offeres a total solution for commercial food testing laboratories. Presenter: Deepali Mohindra, Thermo Fisher Scientific, Sunnyvale, CA, USA, Email: deepali.mohindra@thermofisher.com P-T-009 Scott Krepich , Ramkumar Dhandapani , Phenomenex, Inc., Torrance, CA, USA; Syljohn Estil , Los Angeles County Sanitation District, Whittier, CA, USA; Agustin Pierri , Weck Laboratories, Inc., City of Industry, CA, USA Perfluoroalkyl Substance (PFAS) Analysis in Drinking Water, Sediments, and Food Samples by QuEChERS, SPE, and LC-MS/MS Perfluoroalkyl substances (PFAS) are a class of highly stable synthetic organic compounds used in a wide variety of indus- trial and commercial applications including surface treatment for textiles, packaging materials, non-stick cookware, and fire fighting foams. PFASs are characterized by a hydrophobic fully fluorinated alkyl chain and a hydrophilic functional group. They are persistent in the environment due to the exceptional stabil- ity of the C-F bond. These have been detected throughout the global environment, food products, even human plasma. PFASs are associated with various adverse health effects, they are

bioaccumulative, ubiquitous, and their analysis level require- ments are very low, to account for an expected lifetime of exposure. There are several methods available for the extraction and analysis of PFAS in aqueous samples. However, very few procedures are available for extracting these compounds in solid matrices such as sediments and food samples. Presented are three methods making use of various sample preparation tech- niques for the analysis of PFAS. The methods include, direct inject technique for drinking water, QuEChERS for sediment samples, and QuEChERS followed by SPE for food samples (milk, eggs, and fish tissue). All are validated procedures and makes use of LC-MS/MS. Presenter: Scott Krepich, Phenomenex, Inc., Torrance, CA, USA, Email: scottk@phenomenex.com P-T-010 Josh Messerly , Kai Liu , Ben Pointer , Eurofins, Des Moines, IA, USA Determination of Phytic Acid in Commodities Using Ion Chromatography Conductivity Detection (IC-CD) Phytic acid or phytate are important in agriculture and food science because they are used as a store of phosphorus in plants. This phosphorus is generally not bioavailable to nonru- minant animals. Phosphorus that is not absorbed by the animal can cause phosphorus buildup in waste disposal, and lower feed efficiency. Phytic acid may also chelate certain dietary minerals causing lower absorption and possible deficiency in the animal. A method was developed at Eurofins Nutrition Analysis Center to determine phytic acid in commodities such as corn (maize), soybean, and canola. The method extracts the phytic acid using a dilute hydrochloric acid solution. The sample extracts undergo cleanup using ion exchange cartridges, and then the solutions are analyzed using IC-CD. Results produced by the method during validation show precision between 4-8% RSD and spike recoveries between 99-102%. Presenter: Josh Messerly, Eurofins, Des Moines, IA, USA, Email: joshmesserly@eurofinsus.com P-T-011 Scott Krepich , Phenomenex, Inc., Torrance, CA, USA; Wesley Maguire , Katie Jambor , C. Cerbu , C. Reichmann , C. Call , Columbia Food Laboratories, Portland, OR, USA; Paul Winkler , SCIEX, Golden, CO, USA Expanded Mycotoxin LC-MS/MS Analysis in Cannabis Matrices Mycotoxins are secondary fungal metabolites that can be harmful for human consumption. Some states have requirements for Aflatoxins B1, B2, G1, and G2, and while Oregon does not, secondary metabolites may still be present in cured material, and additional testing is useful for safety and in anticipation of more stringent regulatory requirements. Here we cover a thor- ough LC-MS/MS solution for the analysis of an expanded set of 13 Mycotoxin residues that can be incorporated into pesti- cide screens and rapidly applied to meet changing regulatory requirements. Presenter: Scott Krepich, Phenomenex, Inc., Torrance, CA, USA, Email: scottk@phenomenex.com

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