AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

CANNABIS P-T-012

residues and action limits for each compound varies widely from state to state. Therefore, creating a multiresidue approach encompassing hundreds of pesticides is most practical for meeting all regulations. While both gas (GC) and liquid (LC) chromatography coupled to MS/MS are required for compre- hensive analysis of pesticide residues, this study evaluates a multiresidue approach for GC amenable pesticides in canna- bis. A simple sample preparation approach was taken to extract pesticide residues from cannabis flower which involved shak- ing the ground samples in acetonitrile, followed by dispersive solid phase extraction (dSPE) clean up. The acetonitrile extract was analyzed on a GC-MS/MS system to evaluate sensitiv- ity, linearity and robustness of the system for cannabis matrix. A common detection limit of 10 ng/g (ppb) was evaluated as it is a commonly used standard in pesticide analysis in food products. Linearity over several orders of magnitude were also evaluated with R 2 value greater than 0.99. Robustness was eval- uated using various parameters, including retention time stability, ion ratios, and peak area reproducibility over replicate injec- tions. In summary, a multiresidue approach is being presented for GC-MS/MS analysis of pesticides in cannabis to cover all current U.S. and Canadian regulations. Presenter: Naren Meruva, Waters Corp., Milford, MA, USA, Email: Narendra_Meruva@waters.com P-T-015 Naren Meruva , Kim Tran , Marian Twohig , Michael Young , Waters Corp., Milford, MA, USA; Chris Hudalla , ProVerde Labs, Milford, MA, USA Determination of Pesticides and Mycotoxins in Cannabis Using a Simple Extraction Procedure with dSPE Cleanup and LC-MS/MS A comprehensive testing method which can be used to satisfy state mandated cannabis safety standards for pesticides and mycotoxins is presented. Quantitative methods for residual pesticides and mycotoxins analysis in cannabis should provide reliable coverage and robust methodology for the entire list of analytes mandated by the state. In this study, a comprehensive evaluation of pesticide and mycotoxin analysis in cannabis flower matrix was performed using LC-MS/MS. Ground canna- bis (0.5 g) buds were spiked with acetonitrile spiking solutions containing the pesticide mix. A 5 mL volume of acetonitrile was added. The spiked samples were processed using a Geno Grinder for 3 minutes (1500 rpm). Following centrifugation at 5000 rpm, a 1mL aliquot of the supernatant was added to a dSPE tube, shaken for 1 minute, centrifuged and the superna- tant transferred to a sample vial in preparation for analysis. The LC, MS and data processing methods from Quanpedia method database were utilized to eliminate the need for method development for the state specific pesticide and mycotoxin lists. Overall method performance was evaluated by assessing recovery, matrix suppression, linearity, and sensitivity. Matrix suppression was significantly reduced by dSPE cleanup for many pesticides. Demonstrated method helps meet regulatory require- ments for cannabis pesticide residues and mycotoxins testing. Presenter: Naren Meruva, Waters Corp., Milford, MA, USA, Email: Narendra_Meruva@waters.com

Alicia Stell , Candice Olsson , Brittany Leffler , Candice Cashman , Benedict Liu , CEM Corp., Matthews, NC, USA A New Rapid, Simple, and Efficient Extraction Method for Cannabis With the use of cannabis for both medicinal and recreational purposes becoming legal in numerous states, there is a need to confirm the safety of these products. Confirming that banned pesticides are not present in these products is one of the biggest concerns in this industry. The extraction of pesticides from cannabis can be challenging due to the high levels of interfering chemicals in the plant. Existing techniques do not offer a method that is rapid, simple, and efficient. In this study, a new extraction system, the EDGE, combines the processes of pressurized fluid extraction and dispersive solid phase extraction is explored. This new method offers efficient extraction of pesticides from cannabis in less than 10 minutes in one simple process. Different analytes beyond pesticides, such as cannabinoids, terpenes, mycotoxins can also be extracted with this new method. The EDGE method offers a rapid, simple, and efficient solution for cannabis testing. Presenter: Alicia Stell, CEM Corp., Matthews, NC, USA, Email: alicia.stell@cem.com P-T-013 Ashlee Gerardi , Restek Corp., Bellefonte, PA, USA The Analysis of Mycotoxins in CBD Oils by LC-MS/MS Mycotoxins, secondary metabolites produced by fungi, are among the most toxic contaminants in cannabis and other agricultural products that can cause disease and death in humans and other animals. In the analysis of mycotoxins, using immunoaffinity columns (IACs) to reduce matrix effects and eliminate potential sources of interference for LC-MS/MS anal- ysis is common. When not used, significant matrix interferences have been shown to elute near target mycotoxins resulting in an adverse effect on measured ion ratios. Herein, alternative approaches to IACs including dilute-and-shoot, dispersive sold phase extraction (dSPE) and pass-through SPE are evaluated and applied to the analysis of aflatoxin B 1 , aflatoxin B 2 , aflatoxin G 1 , aflatoxin G 2 , and ochratoxin A in commercially available CBD oils by LC-MS/MS. Presenter: Ashlee Gerardi, Restek Corp., Bellefonte, PA, USA, Email: ashlee.gerardi@restek.com P-T-014 Kim Tran , Kari Organtini , Pete Hancock , Naren Meruva , Kenneth Rosnack , Waters Corp., Milford, MA, USA A Multiresidue Approach to Pesticide Screening in Cannabis Using GC-MS/MS The objective of this study is to demonstrate a multi-resi- due analysis of GC amenable pesticides in cannabis matrix using GC-MS/MS. In the United States the list of pesticide

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