AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

P-T-016 Naren Meruva , Catharine Layton , Andy Aubin , Waters Corp., Milford, MA, USA Comparison of HPLC and UPLC Methods for the Determination of Cannabis Potency High Performance Liquid Chromatography (HPLC) is the preferred methodology for potency determination as it can identify and measure structurally similar cannabinoids and their different forms (e.g. free form and corresponding acid forms of THC and CBD) in a single analysis. In this study we will compare the fit-for-purpose HPLC method to a higher throughput Ultra Performance Liquid Chromatography (UPLC) method for quantitation of 16 cannabinoids in plant material and concen- trates to ensure quality and safety of cannabis products. As the legalization of cannabis for both medicinal and recreational use continues to advance, the need for simple, reliable analytical methods for the analysis of these products is desired by grow- ers, extract producers, regulators and consumers. Conversion of the HPLC method (25 min) to UPLC method (10 min) provides a greater than two-fold increase in testing productivity while maintaining the linearity, selectivity, and suitability observed in the HPLC separation. Presenter: Naren Meruva, Waters Corp., Milford, MA, USA, Email: Narendra_Meruva@waters.com P-T-017 Toby Astill , PerkinElmer, Inc., Woodbridge, ON, Canada High-throughput Automated LC/MS Pesticide Residue Analysis of Cannabis Samples to Meet Canadian and California Regulatory Requirements Cannabis testing laboratories face increasing demands for faster turnaround times; to address this PerkinElmer will present a brand-new analytical solution, with full automation, that allows laboratories to run more samples in a workday. The fully auto- mated platform prepares cannabis samples, on a robust liquid handling capability, and completes the testing requirements on a novel LC/MS instrument with ESI and APCI ion sources to meet State Testing requirements. Sample throughput, detection limits for California and Canada will be highlighted, and data report- ing will be highlighted in this session. Presenter: Toby Astill, PerkinElmer, Inc., Woodbridge, ON, Canada, Email: toby.astill@perkinelmer.com P-T-018 Aihua Liu , Daniel Taylor , Spencer Carter , Dyad Labs, Salt Lake City, UT, USA A Fast, Sensitive, Comprehensive, and Accurate Quantitative Assay of 16 Cannabinoids in Different Matrix by LC-MS/MS In 2018, AOAC has published a method to quantify 10 cannabi- noids (CBD) with the range of 0.500-10.0 µg/mL in cannabis. However, there are more than 100 CBD isolated from cannabis in addition to above 10 CBD. Furthermore, CBD have been legally and widely used for more and more products. Thus, a

more sensitive potency method with more CBD is needed. Dyad Labs has developed a fast, sensitive, accurate and comprehen- sive quantitative method for 16 major cannabinoids in different matrix using LC-MS/MS. During method development, the stability of 16 major CBD have been comprehensively studied. Different parameters of pH, temperature and light exposure were investigated, and the data indicated that most CBD are more stable under acid condition and sensitive to light, which suggests that CBD need to be stored and processed under yellow light and appropriate pH. The extract was then injected on a Raptor ARC-C18 column with formic acid/water/methanol as mobile phases and AB Sciex 5500 triple quadrupole mass spectrom- etry with using MRM as detector. The method can be used on different matrices, including CBD drop in coconut oil, CBD drop in water, CBD tablet and CBD capsule. We have successfully developed an assay covering the range of 0.00500-0.500 µg/mL with acceptable specificity, accuracy and precision (RSD<15%). This is the first published fast, sensitive and compre- hensive assay for quantitation of 16 cannabinoids in different matrix using LC-MS/MS. Presenter: Aihua Liu, Dyad Labs, Salt Lake City, UT, USA, Email: aihua.liu@dyadlabs.com P-T-019 Aihua Liu , Paul Kelly , Daniel Taylor , Spencer Carter , Dyad Labs, Salt Lake City, UT, USA A Fast, Comprehensive and Accurate Quantitative Assay of 16 Cannabinoids in Different Matrix by UPLC-PDA Cannabis is a psychoactive drug from the cannabis plant used for medical or recreational purposes. Increased legalization of cannabis for medial and recreational use substantiates the need for a standardized robust and reproducible method for quantitation of active cannabinoids (CBD). In 2018, AOAC has published a method to quantify 10 CBD. However, there are more than 100 cannabinoids isolated from cannabis in addi- tion to above 10 cannabis. Dyad Labs has developed a fast, comprehensive assay to quantify 16 major CBD in different matrix using UPLC-DAD. During method development, the chro- matographic resolution of these 16 cannabinoids became very challenging. Delta-9-THC/CBNA and CBGA/CBG coeluted on different columns and mobile phases. The Raptor ARC-C18 column with mobile phases of formic acid/ammonia formate/ water/methanol/acetonitrile can separate all 16 CBD. Since we also target to develop more sensitive method on LC-MS/MS platform, the method range in this paper is 1.00-20.0 µg/mL for samples with high specification. The run time is 12 mins with using 220 nm as the detection wavelength. Different matrix, including CBD drop in coconut oil, CBD drop in water, CBD tablet and CBD capsule, were evaluated. The method was successfully developed over the range of 1.00-20.0 µg/mL with acceptable specificity, accuracy and precision (RSD<5%). This is the first published fast, comprehensive and accurate assay for quantita- tion of 16 CBD in different matrix using UPLC-PDA. Presenter: Aihua Liu, Dyad Labs, Salt Lake City, UT, USA, Email: aihua.liu@dyadlabs.com

WWW.AOAC.ORG 301.924.7077 63