AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

P-T-025 Mark Waksmonski , Herb Potthoff , Shimadzu Scientific Instruments, Inc., Columbia, MD, USA; Kevin Albertson , Act Laboratories, Toledo, OH, USA A Method Development Process for Analysis of Metals in Cannabis by ICPMS Since metals testing requirements for metals in Cannabis are regulated at the state level, each state’s testing regulations may differ. These differences may include what metals are tested for, the permissible concentrations, or other method specific require- ments. Therefore, it is difficult to have a single testing procedure that applies to testing labs in all states. Here we will lay out a common- sense approach for these labs to develop an ICP-MS method for cannabis analysis that is appropriate to their state requirements. Presenter: Mark Waksmonski, Shimadzu Scientific Instruments, Inc., Columbia, MD, USA, Email: mawaksmonski@shimadzu.com P-T-026 Kerri-Ann Blake , Metrohm USA, Riverview, FL, USA Comparative Study of Moisture Analysis Techniques on Cannabis As the Cannabis industry grows, testing for residual solvents, contamination, terpenes, and potency is in high demand. One of the parameters of the potency calculation is moisture, which is often determined by loss on drying. Unfortunately, this technique is not specific to moisture and the loss of any volatile compo- nents, such as terpene, will be incorrectly classified as moisture. Karl Fischer titration is the only chemically specific test for moisture. It requires less than 0.5 grams of sample and produces no odor. Attend this session to learn how Karl Fischer titration can be used to determine moisture in cannabis flowers, oils and various related products. Presenter: Kerri-Ann Blake, Metrohm USA, Riverview, FL, USA, Email: kerriann.blake@metrohmusa.com P-T-027 Robert Clifford , Jeff Dahl , Craig Young , Max Wang , Paul Winkler , Andrew Fornadel , Rachel Lieberman , Nicole Lock , Scott Kuzdzal , Shimadzu Scientific Instruments, Inc., Columbia, MD, USA A Closer Look at the Modern Cannabis QC Testing Laboratory Mass Spectrometry plays a major role in the determination of cannabis product contamination — from mycotoxins, aflatoxins and pesticides, to residual solvents and heavy metals. We herein present cannabis contaminomics applications along with exam- ple data, as well as examine the best analytical instrumentation technologies. Presenter: Robert Clifford, Shimadzu Scientific Instruments, Inc., Columbia, MD, USA, Email: rhclifford@shimadzu.com

are anticipated to bolster this growing industry in the coming years. New regulations of heavy metals for cannabis-based products will come into force to manage this emerging market. In this work, we explore and discuss the applicability of the Shimadzu inductively coupled plasma–mass spectrometer 2030 (Shimadzu ICPMS-2030) to the qualification and quantifica- tion of heavy metals in cannabis-based products. Specifically, 17 different heavy metals in typical cannabis-based products including cookie, chocolate, gummy bear, olive oil, hard candy, butter and hops were extracted and determined with a Shimadzu ICPMS-2030. Spiked samples with known concentrations along with original samples were also analyzed to calculate recoveries to evaluate the precision and accuracy of this method. The results indicate that Shimadzu ICPMS-2030 provides excellent sensi- tivity, precision, accuracy, tolerance and fast time response to meet and exceed compliance with regulations on heavy metals in complex matrices of cannabis-based products. Presenter: Jonathan Peters, Shimadzu Scientific Instruments, Inc., Columbia, MD, USA, Email: jdpeters@shimadzu.com P-T-024 Leyda Lugo-Morales , Alan Owens , Nicole Lock , Andy Sandy , Richard Karbowski , Christopher McGuire , Sono Yem , Vikki Johnson , Herb Potthoff , Robert Clifford , Shimadzu Scientific Instruments, Inc., Columbia, MD, USA Selective and Sensitive Analysis of 20 Category I and II Residual Solvents in Cannabis Products Using Headspace-GC/MS Analysis of residual solvents by gas chromatography and gas chromatography/mass spectrometry (GC/MS) coupled with headspace sampling is a standard analytical technique that applies in many fields such as pharmaceuticals, food packaging, forensics, and cosmetics. In such fields, standardized proto- cols exist for sample preparation and analysis methodology. A steady increase in popularity of cannabis concentrate products has been observed, which has led to the establishment of new regulations to monitor the level of residual solvents present in these products. In the cannabis industry, many different solvents are used in the extraction process, each one classified based on the level of toxicity. Regulated action levels vary based on the hazard classification of residual solvents, some requiring a high sensitivity method, such as GC/MS for their low-level quantitation. Also, sample preparation is difficult with processed cannabis matrices due to their lack of solubility and limitation of available non-interferent diluent solvents. The ideal way for test- ing is direct analysis of the sample in a headspace vial without any sample preparation involved. Furthermore, other method development challenges exist, such as co-elution of several residual solvent analytes or mass-to-charge interferences from the environment. Finding an acceptable balance between proper separation for unequivocal identification and quantitation, and reasonable analysis throughput time can be complicated. Presenter: Leyda Lugo-Morales, Shimadzu Scientific Instruments, Inc., Columbia, MD, USA, Email: lzlugo-morales@shimadzu.com

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