AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

P-T-028 Vikki Johnson , Jordon Frost , Herb Potthoff , Shimadzu

P-T-030 Jan Knoop , Johannes Hesper , Uwe Oppermann , Shimadzu Europa GmbH, Duisburg, Germany Analysis of Heavy Metal Contaminants in Hemp and Cannabis Flower Using ICP-Mass Spectrometry The recent changes in legislation in the European Community and other European countries has opened up the cultivation and sale of cannabis and related products in a variety of states and municipalities. With the availability of hemp and cannabis as commercial products such as tea, oil, cannabis drinks and more comes the need for analysis and regulation of potency, pesticides, biological contaminants, and heavy metals, among others. The concentration of heavy metals in plants that are intended for consumption is of concern due to the potentially hazardous effects of these metals related to their toxicity. As they grow, plants can bioaccumulate metals in their tissues that originate from the soil and water in which they are grown. These metals may originate naturally in soils and water as a result of the mineral content of the soil or source of the water, or they may be artificially introduced in the form of fertilizers, pesticides, herbicides, and fungicides commonly applied to increase crop yields. Some of these metals contained in plants have beneficial metabolic uses, such as iron in beans and leafy greens, whereas others, such as lead, can have deleterious effects including toxicity and carcinogenicity. Here, we explore and discuss the applicability of the Shimadzu ICP- mass spectrometer ICPMS- 2030 to the detection of the “Big Four” heavy metals (i.e., As, Cd, Hg, and Pb) in digested hemp and cannabis flower samples. Presenter: Uwe Oppermann, Shimadzu Europa GmbH, Duisburg, Germany, Email: uo@shimadzu.eu P-T-031 Lukas Vaclavik , Eurofins Food Integrity & Innovation, Harrogate, United Kingdom; Frantisek Benes , Marie Fenclova , Jiri Hricko , Ales Krmela , Veronika Svobodova , Jana Hajslova , University of Chemistry and Technology, Prague, Czech Republic; Katerina Mastovska , Eurofins Food Integrity & Innovation, Madison, WI, USA Determination of Cannabinoids in Plant Materials, Oils and Concentrates Using UHPLC–DAD: Single Laboratory Validation for AOAC First Action Official Method Consideration A liquid chromatography–diode array detection (LC–DAD) method was validated for quantification of twelve major canna- binoids in Cannabis dried plant materials, oils, and concentrates. The method met AOAC Standard Method Performance Requirements (SMPRs 2017.001 and 2017.002) for quantita- tive analysis of cannabinoids in Cannabis concentrates and Cannabis dried plant materials. It was approved AOAC First Action Official method 2018.11. The limits of quantification (LOQs) were in the range 0.003–0.10% (w/w), depending on the analyte and matrix. Spike recoveries were between 96.7–101.3% with relative standard deviations (RSDs) ≤ 2.3%. Precision expressed as repeatability (RSD r ) and intermedi- ate precision (RSD INT ) was within 0.3–4.8% and 1.1–5.1%, respectively. The chromatographic separation conditions used in this versatile method are compatible with both DAD-UV and mass spectrometric detection. During method validation,

Scientific Instruments, Inc., Columbia, MD, USA Simplified Terpene Profiling by GC/MS

Terpene and terpenoid compounds are naturally occurring aromatic compounds that give cannabis its unique flavor and fragrance. Aside from their aromatic properties, terpenes have advantageous health benefits. They also have a syner- gistic relationship with cannabinoids, which further enhance the therapeutic effect of THC. Cannabis has over 140 terpene components, many of which are of medicinal interest. The concentration of individual terpenes varies by strain, can be anywhere from 0.1 to 1.5% of its total dry weight, and can vary depending on harvest time, drying and storage conditions. Recent proliferation of new terpene profiling methods can be attributed to the ever-increasing state legalization of cannabis use. Due to the uniqueness of terpene profiles, they can be used by cultivators as a “fingerprint” to partially ID the specific strain in question. This poster describes the analysis of several strains of cannabis for 21 terpenes using gas chromatography/mass spectrometry (GC/MS) with headspace injection. Presenter: Vikki Johnson, Shimadzu Scientific Instruments, Inc., Columbia, MD, USA, Email: vijohnson@shimadzu.com P-T-029 Gesa Schad , Uwe Oppermann , Philipp Jochems , Robert Ludwig , Vadim Kraft , Shimadzu Europa GmbH, Duisburg, Germany Determination of Cannabidiol and Additional Cannabinoid Content in Hemp Tea Cannabis contains more than 500 unique compounds, including over 80 chemical alkaloids known as cannabinoids. Numerous health benefits have been reported that are attributed to their pharmacological characteristics, which allow for use as medi- cal treatment. They can affect physiological processes, such as inflammation, pain perception and seizures, which is a reason for the growing interest in “Medical Cannabis” [1, 2]. While use of cannabis for medicinal purposes is still subject to a lot of debate in Europe, hemp products containing < 0.3% of the psychoactive compound d9-tetrahydrocannabinol (THC) have always been legal in most countries. With the growing interest in the cannabis plant, the market for cannabidiol (CBD) containing food and cosmetics products is also increasing. Quantification of canna- binoids is essential for the accurate labeling of hemp products, for quality control, as well as to establish legality with regards to the THC content. In this work, High Performance Liquid Chromatography (HPLC) is the method of choice for analysis of cannabinoid content in different CBD rich hemp tea samples. The HPLC-UV method used provides good linearity, low limit of detection, as well as high precision of retention time and peak area for the cannabinoids under investigation. Presenter: Uwe Oppermann, Shimadzu Europa GmbH, Duisburg, Germany, Email: uo@shimadzu.eu

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