AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

80-120%. This method is reliable for the quantitation of OTA and suitable for quick analysis for quality control purpose. Presenter: Congmei Cao, Herbalife Nutrition, Torrance, CA, USA, Email: congmeica@herbalife.com P-T-058 Charikleia Tsaridou , Sofia Iliopoulou , Nikolaos Natsaridis , Antonios Skliris , Georgios Papageorgiou , Antonios Ntantasios , Sotirios Athanasiou , Sotiria Drakouli , R&D Department, Prognosis Biotech S.A., Larissa, Greece Validation of a 5-Minute Lateral Flow Test for the Quantification of Ochratoxin A in Wine Samples In the analysis of wine, there are several ELISA tests that either detect, semi-quantify or quantify Ochratoxin A (OTA) (EU limit: 2 μg/kg) with a total procedure time of 60-90 min. Regarding the Lateral Flow (LF) technology, the quantification of OTA in wine is quite limited with a procedure time of 10 min and most of the products available are only qualitative. In addition, intensely colored matrices like wine require special pretreatment. The aim of this work was to determine the OTA levels in spiked wine samples with a new, simple and innovative quantitative 5-minute LF assay, comparing the recovery results with ELISA. The LF Symmetric Ochratoxin Wine (B6148, Lot B6148002) and the ELISA Bio-shield Ochratoxin Wine (B6196, Lot B6196004) (ProGnosis Biotech S.A.) were used. Considering the physical and chemical properties of wine, both methods use a dilution normalization without previous treatment. Twelve OTA-free wine samples originating from different varieties were chosen and spiked with OTA. Reference Materials (RMs) were also used and the recovery of all samples was calculated. Using the 5-minute Symmetric LF assay, the recovery and CV% of the samples were identical with ELISA’s. The level of 1.5 ppb was easy to be detected visually, making the assay suitable for a qualitative test too. This innovative LF assay constitutes the most simple, rapid and accurate tool in the quantification of OTA in wine samples, providing results comparable to those of a quantitative ELISA. Presenter: Georgios Papageorgiou, R&D Department, Prognosis Biotech S.A., Larissa, Greece, Email: g.papageorgiou@prognosis-biotech.com P-T-059 Alex Levine , Toshi Ono , Nacalai Tesque, Inc., San Diego, CA, USA; Tsunehisa Hirose , Nacalai Tesque, Inc., Kyoto, Japan LC-MS Analysis of Mycotoxins-Unique Stationary Phases for Alternative Selectivity Analysis of mycotoxins is becoming increasingly difficult as the list of regulated toxins grows. One of the most common ways to detect mycotoxins is by high performance liquid chromatogra- phy (HPLC) in tandem with a mass spectrometer (LC-MS). The challenges in mycotoxin analysis by HPLC are not only the sepa- ration and identification of many toxins in a single run, but the resolution of these toxins from matrixes compounds. In addition to developing methods for standard agricultural crops, industries such as the developing cannabis/hemp market require new strategies to contend with the complexity of their matrixes. One way in which resolution of analytes can be improved in HPLC

and LC-MS is by alternative column chemistries. Presented here is the simultaneous LC-MS analysis of 12 mycotoxins on different column stationary phases, emphasizing differences in column selectivity. Presenter: Alex Levine, Nacalai Tesque, Inc., San Diego, CA, USA, Email: alex@nacalaiusa.com P-T-060 Konstantina Badra , Evanthia Angeli , Charikleia Tsaridou , Dimitrios Foulos , Georgios Papageorgiou , Antonios Ntantasios , Sotiria Drakouli , Sotirios Athanasiou , R&D Department, Prognosis Biotech S.A., Larissa, Greece Mycotoxin-Free 5-Minute ELISA System Using One Standard and Precalibrated Curve for the Quantification of Mycotoxins The rapid quantification of Aflatoxins, Deoxynivalenol (DON), Zearalenone (ZON), Ochratoxin Α (OTA), Fumonisins, T-2/ HT-2 in grains, without handling harmful toxins, constitutes a challenge. The development of accurate and repeatable toxin-free rapid ELISA, having low LOD, LOQ and CV%, could be an essential tool for mycotoxin quantification. Aim of this study was to evaluate the recovery levels of mycotoxins, in grains, using a 5-min ELISA with one toxin-free standard after a single extraction. The levels of AFB1, Total Aflatoxin, DON, ZON, OTA, Fumonisins, T-2/HT-2 were determined using the One Standard 5-min ELISA (Prognosis Biotech S.A.) Bio-Shield: One Standard B1 (B4948-005), One Standard Total (B4348- 009), One Standard DON (B4548-006), One Standard ZON (B4448-005), One Standard Ochratoxin (B4248-004), One Standard Fumonisin (B4748-003) and One Standard T-2/ HT-2 (B4848-005), respectively. All methods were used with or without standards. Toxin-free samples were spiked at three levels (including the LOQ) with a multi-toxin solution and extracted with methanol 70%. FAPAS Reference materials were also tested. The recovery and CV% in all samples were acceptable with or without the use of standards. The LOQ did not show significant difference between the paired methods, as well. The innovative Mycotoxin-free 5-min ELISA, using one standard, a precalibrated curve and one single extraction, is effortless and accurate, providing unique advantages in the quantification of mycotoxins. Presenter: Georgios Papageorgiou, R&D Department, Prognosis Biotech S.A., Larissa, Greece, Email: g.papageorgiou@prognosis-biotech.com P-T-061 Antonios Skliris , Sotiria Drakouli , Maria Tziortziou , Sofia Iliopoulou , Georgios Papageorgiou , Antonios Ntantasios , Sotirios Athanasiou , R&D Department, Prognosis Biotech S.A., Larissa, Greece One Step Multitoxin Aqueous Extraction for the Quantification of All Mycotoxins Using Symmetric Lateral Flow Technology The use of state-of-the-art features for the quantification of all the major mycotoxins (Aflatoxins, Deoxynivalenol (DON), Zearalenone (ZON), Ochratoxin Α (OTA), Fumonisins, T-2/ HT-2) in grains, constitutes a challenge. An aqueous extraction

74 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL