AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

P-T-063 Evangelia Nikolopoulou , Panagiota Tsikritsi , Konstantina Badra , Charikleia Tsaridou , Georgios Papageorgiou , Antonios Ntantasios , Sotiria Drakouli , Sotirios Athanasiou , R&D Department, Prognosis Biotech S.A., Larissa, Greece Validation of an Ultra-Fast 5-Minute ELISA System for the Quantification of Mycotoxins The rapid quantification of Aflatoxins (AF), Deoxynivalenol (DON), Zearalenone (ZON), Ochratoxin Α (OTA), Fumonisins, T-2/HT-2) in grains, using a single extract, constitutes a chal- lenge. A time-saving assay with high accuracy and repeatability, having also low LOD, LOQ and CV%, can be a valuable tool in mycotoxin analysis. Consequently, the use of a single extract in a 5-minute next generation ELISA is important for the quantification of mycotoxins. The aim of this study was to evaluate the recovery levels of mycotoxins in spiked samples and Reference Materials (RMs), using a 5-minute ELISA with one single extract. The levels of AFB1, Total Aflatoxin, DON, ZON, OTA, Fumonisins, T-2/HT-2 toxins were determined using the 5-minute ELISA (Prognosis Biotech S.A) Bio-Shield B1 5/ B5048-004, Total 5/B5148-005, DON 5/B5248-005, ZON 5/B5348-003, Ochratoxin 5/B5448-003, FUMONISIN 5/ B5548-004 and T2/H-T2 5/B5648-004, respectively. It was used a single extraction with methanol 70% in all 5-minute methods. Mycotoxin-free samples were spiked with a multitoxin solution and RMs were also analyzed. All recovery levels in the spiked samples were acceptable. The results were also confirmed by analyzing the RMs. The CV% of all samples were also within an acceptable range. The innovative 5-minute Bio-Shield ELISA technology gives acceptable recovery and CV% using a single extraction. The ultra-fast ELISA is effortless and accurate, provid- ing unique advantages in terms of time-saving. Presenter: Georgios Papageorgiou, R&D Department, Prognosis Biotech S.A., Larissa, Greece, Email: g.papageorgiou@prognosis-biotech.com P-T-064 Caly Zmijewski , Mérieux NutriSciences Corp., Chicago, IL, USA; Mike Erickson , Brian Oliveira , Mérieux NutriSciences Corp., Salida, CA, United States; Elizabeth Manning , Martin Sibum , R-Biopharm AG, Glasgow, United Kingdom Automation for the Analysis of Aflatoxin in Nuts Reduces Analysis Time, Improves Workflow, and Maintains USDA Criteria for Export Using an Online Immunoaffinity Clean-Up Cartridge Tree nuts and peanuts are valuable, high volume commodities for the USA domestic and export markets. All types of nuts are susceptible to contamination with aflatoxins. Regulations are in place for maximum levels permitted in these commodities. Laboratories must demonstrate that they can comply with these requirements through the validation of analytical methods. The high volume of the various types of nuts processed, particu- larly at peak seasonal times, places a demand on the time and resources found in a typical laboratory. Sample extraction followed by automated immunoaffinity clean-up performed on the RIDA ® CREST integrated HPLC system with IMMUNOPREP ® ONLINE cartridges ensures that a higher number of samples

could also eliminate the adverse effects of organic solvents. A Lateral Flow (LF) assay with a multitoxin aqueous extraction and high accuracy and repeatability, having also low LOD, LOQ and CV%, could be an essential tool in mycotoxin analysis. The aim of this study was to evaluate the recovery levels of mycotoxins in corn and animal feed, using one single aqueous extraction and LF Symmetric Technology. The mycotoxin levels were determined by the 5-minute Symmetric Green LF assays (Prognosis Biotech S.A.). Total Aflatoxin, DON, ZON, OTA, Fumonisins, T-2/ HT-2 were determined using the Symmetric Total Green 0-30/ S3448-004, Ochratoxin Green / S6048-004, DON Green/ S4048-008, ZON Green/S5048-004, Fumonisin Green/ S7048-005 and T-2/HT-2 Green/S8048-004, respectively. Different mycotoxin FAPAS Reference Materials were used. The recovery levels and CV% were acceptable and the results were in agreement with those of different FAPAS multi mycotoxin Ring Tests. For the first time, a single aqueous extraction can be used for all mycotoxins, providing unique advantages in terms of cost and time-saving while Symmetric Technology signifies the transi- tion of LF sticks from being a low-esteemed screening tool into a reliable confirmatory method. Presenter: Georgios Papageorgiou, R&D Department, Prognosis Biotech S.A., Larissa, Greece, Email: g.papageorgiou@prognosis-biotech.com P-T-062 Mike Awada , Anna Muhich , Kallie Strong , Brittany Graf , Groviv, Vineyard, UT, USA Simultaneous Determination of Animal Feed Mycotoxins Using LC-QTOF-MS/MS Mycotoxins are naturally occurring fungal secondary metab- olites that can cause adverse health effects in animals and humans. Due to the diverse chemical and physical structure of mycotoxins and their different regulatory limits, comprehensive mycotoxin analysis is both challenging and time consuming. Current mycotoxin compendial methods mainly involve the determination of single compounds or classes thereof, but it would be preferable to determine most mycotoxins by routine analysis in one single extract. The aim of this study was to develop, optimize, and validate a simple and sensitive method for the quantitative analysis of multiple regulated mycotoxins in wheatgrass, barleygrass, and their seeds. Samples were extracted using CHCl 3 :EtOH (80%:20%), and the quantitative analysis of four aflatoxins, zearalenone, deoxynivalenol, 3- and 15-acetyldeoxynivalenol, and T-2 toxin was performed using an ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC- QTOF–MS/MS). Matrix-adjusted calibration curves for all analytes showed good linearity (R 2 > 0.99), repeatability (CV < 10%), accuracy (80-110%), and recovery (85-105%). The limit of quantification for the aflatoxins was 1.0 µg/kg −1 , for DONs 10-50 µg/kg −1 , for T2 toxin 10 µg/kg −1 , and for zear- alenone 25 µg/kg −1 . This procedure will satisfy the regulatory European EFSA and U.S. FDA requirements for analysis of these mycotoxins. Presenter: Anna Muhich, Groviv, Vineyard, UT, USA, Email: amuhich@groviv.com

WWW.AOAC.ORG 301.924.7077 75