AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

76 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL are processed, while maintaining method accuracy, precision, and sensitivity. Presenter: Elizabeth Manning, R-Biopharm AG, Glasgow, United Kingdom, Email: elizabeth@r-biopharmrhone.com GENERAL METHODS, QUALITY ASSURANCE AND ACCREDITATION P-T-065 Nina Nielsen , NMKL, Kgs Lyngby, Denmark NMKL: Nordic Committee on Food Analysis Nordic Committee on Food Analysis (NMKL), is a network for chemists, microbiologists and sensory analysts working in food laboratories, food industry, research institutions and in food control authorities. Members of NMKL are chemical and micro- biological experts appointed by the national committees from the five Nordic countries. NMKL has interested parties from over 40 countries and cooperates with several international organiza- tions. NMKL elaborates and collaboratively validate analytical chemical, microbiological and sensory methods for analysis of foods and feeds. NMKL elaborates procedures for laborato- ries and users of their services on sampling, method validation, measurement uncertainty etc. NMKL also arranges courses and workshops to enable increased knowledge and technical skills of analytical scientists. NMKL holds the secretariat of NordVal International. NordVal International performs a third-party review and certifies proprietary chemical and microbiological methods. The members of NordVal International are experts appointed by the national committees. They have to be independent and cannot have any special interest in the production/distribution of test kits, special reagents or instruments. NordVal International offers a user-friendly validation protocol, scientific confirmation policies, specified acceptance criteria, independent and rapid approval procedures and guidance in the validation process. Presenter: Nina Nielsen, NMKL, Kgs Lyngby, Denmark, Email: nsni@food.dtu.dk P-T-066 Navya Jonnalagadda , Ryan Malone , Trilogy Analytical Laboratory, Hyderabad, India; Subramanian Sankaran , Ministry of Textiles, Mumbai, India; Shivani Desai , R-Biopharm Neugen Pvt Ltd, Hyderabad, India A Study on Performance Evaluation of Participants in Proficiency Testing Rounds, Evaluating Different Outlier Removal Methods and Standard Deviation for Proficiency Assessment Calculations Participants in proficiency testing (PT) rounds range from novice to expert; this disparity can create large deviations between participant results. If the variations are unreasonably wide, the Standard Deviation for Proficiency Assessment (SDPA) assigned by consensus will be grossly overestimated. In order to prevent an unreasonably wide SDPA, outlier removal approaches were evaluated including effects on z -score. The assigned value and SDPA were calculated by 3 approaches including robust statis- tical analysis without outlier removal, double Grubb’s test outlier removal, and a combination of blunder and outlier removal by maximal acceptable permissible error and double Grubb’s test,

respectively. The SDPA was also calculated with the Horwitz- Thompson equation for each of the assigned values as a control. The z -score for participants were calculated by applying the distinct SDPAs with corresponding assigned value. Using the maximal permissible error technique for blunder elimination followed by outlier removal with double Grubb’s test, proved to provide the most accurate performance evaluation for limited participant PT rounds. This data most suitably represented statisti- cal tendencies as per normal distribution supporting the basis of z -score performance interpretation. Presenter: Navya Jonnalagadda, Trilogy Analytical Laboratory, Hyderabad, India, Email: navya@trilogylab.in P-T-067 Terri Christison , Jeffrey Rohrer , Thermo Fisher Scientific, Sunnyvale, CA, USA Determination of Brominated Vegetable Oils in Beverages Using Pyrolytic Combustion Coupled to Ion Chromatography Brominated vegetable oil (BVO), is a food additive used to emulsify citrus flavoring and provide a stable mixture in carbonated beverages. BVO is a heterogenous fatty acid composed of plant-based oils which are brominated at the previ- ously unsaturated bonds. Analytical methods are needed for BVO determinations to maintain the beverage quality and to meet labeling requirements. However, the determinations are challenging because BVO exists as a non-polar suspension in an ionic sample matrix. Pyrolytic combustion ion chromatography is an ideal approach to eliminate the sample matrix and increase sample homogeneity. In this application, BVO was deter- mined in beverage samples by pyrolyzing a 50 µL aliquot at 1000C under inert atmosphere and combusted with oxygen, water, and peroxide to obtain bromide. Bromide and other ions in the collected aqueous sample are separated by anion-exchange chromatography on a 4 x 150 column using electrolytically generated 23 mM KOH at 1.0 mL/min and detected by suppressed conductivity. Using overlap mode for the 5-min combustion, the total run time is 13 min. BVO was determined in three carbonated beverage and two ginger beer samples. Bromide from total BVO was found at expected levels in one of the carbonated beverages and at trace levels in another carbonated beverage. The method had good accuracy with recoveries within the 80-120% and good repro- ducibility with <5% RSDs. Presenter: Terri Christison, Thermo Fisher Scientific, Sunnyvale, CA, USA, Email: terri.christison@thermofisher.com P-T-068 Ioannis Vrasidas , Geralt Ten Kate , Katerina Koureta , Peter Sanders , Jeroen van Soest , Eurofins Food, Feed & Water Testing Netherlands, Heerenveen, Netherlands Development and Validation of a Method for 2’-Fucosyllactose Determination in Infant Formula, Premixes, and Raw Material Human Milk Oligosaccharides (HMO) represent approximately 20% of the total carbohydrate content in human milk and are its third largest solid component. Studies have shown the impor- tance of HMO during the newborn’s development. Antiadhesive