AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

than those of IPC. Amplifications were also observed from both the standard DNA and IPC on another duplex PCR. These results suggested that higher amount of genome and standard DNA did not inhibit the amplification of IPC in which a total amount of 10 copies were successfully detected. We expect that the IPC, prepared by the novel reference material, can be used to determine the true positive and negative results in a real-time PCR analysis. Therefore, various characteristics of multiplex PCR will be observed. Presenter: Mari Onishi, FASMAC Co., Ltd, Atsugi, Japan, Email: omari@fasmac.co.jp P-T-079 Jianhui Li , Defeng Huang , Yuhong Qin , Waters Corp., Beijing, China; Fang Wen , Ministry of Agriculture, Beijing, China; Jinchuan Yang , Waters Corp., Milford, MA, USA Rapid Determination of Lactulose in Dairy Products Using Ultraperformance Convergence Chromatography Coupled with Mass Spectrometry Lactose is the main carbohydrate in milk. Different processing technology, for example, high temperature sterilization, could lead to the conversion of lactose into lactulose. Lactulose is the most widely studied marker for distinction of heat-treated milk and for evaluating the heat load to which milk was subjected. In addition, sucrose may be added into processed foods, such as yogurt and milk beverage to improve the flavor of prod- ucts. Enzymatic, GC and HPLC methods have been applied to detection lactulose in milk. The major disadvantages of these methods are laborious, time consuming and less sensitive. One of the main problems in measuring lactulose in dairy products is the separation of lactulose from lactose and sucrose using HPLC. Also, the amount of sucrose and lactose are more than two orders of magnitude larger than that of lactulose. Here we present a rapid and sensitive quantification of lactulose in dairy products based on ultra-performance convergence chroma- tography (UPC 2 ) coupled with an ACQUITY QDa. The target analytes were separated well on a Waters ACQUITY UPC 2 Torus DEA column based on MS chromatography using selected ion recording mode. Calibration curve of lactulose was quadratic (r 2 ≥ 0.99) within the range of 0.1 to 10.0 mg L -1 . The relative stan- dard deviation (RSD, n = 6, 10.0 mg L -1 ) of retention time is 0.2% and that of peak area is 0.5%. The limits of detection and quanti- fication of the lactulose were 0.75 and 2.5 mg L -1 , respectively. Presenter: Jinchuan Yang, Waters Corp., Milford, MA, USA, Email: Jinchuan_yang@waters.com P-T-080 Lawrence Pacquette , Abbott Laboratories, Columbus, OH, USA; Jenny Nelson , Agilent Technologies, Inc., Santa Clara, CA, USA Simultaneous Determination of Iodine and Bromine Species in Infant Formula and Nutritional Products by HPLC-ICP-MS A fast and sensitive method for the analysis of iodine and bromine species in infant formula was developed using high- performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The 4 species of interest were I − , IO 3 − , Br − , and BrO 3 − . All 4 species had baseline

separated in less than 6.5 min using anion exchange resin column. Calibration for the four species was obtained from 0.1 up to 100 ppb with linearity better than 0.9999 in all cases. The limits of detection for I − , IO 3 − , Br − , and BrO 3 − were all less than 0.6 ug/ kg respectively. The method was used to determine the four halogen species in a NIST 1849a (Infant/Adult Nutritional Formula) standard reference material and four commercially available infant formula products from the United States and China. To test the suitability of the method for the accurate determination of low concentrations of the four species in infant formula samples, a spike recovery test was carried out at 20 and 40 µg/kg. Total elemental determinations of iodine and bromine were also performed using a triple quadrupole ICP-MS, without HPLC. Presenter: Lawrence Pacquette, Abbott Laboratories, Columbus, OH, USA, Email: lawrence.pacquette@abbott.com P-T-081 Michie Hashimoto , Ricoh Co., Ltd, Kawasaki, Japan; Mari Onishi , Ian Riztyan , Satoshi Futo , Fasmac Co., Ltd, Atsugi, Japan; Reona Takabatake , Kazumi Kitta , National Agriculture and Food Research Organization, Tsukuba, Japan Novel DNA Reference Material by Bioprinting VIII: Method to Indicate PCR Inhibition and Quality of DNA Samples PCR inhibitors contains chemical substances which can cause lack of sensitivity and false negative results. It can be found in DNA samples in which potential PCR inhibitor substances are remained after DNA extraction process. Quality of DNA samples such as DNA purification is still considered as param- eter for successful PCR reactions. However, it is difficult to determine a standard for DNA samples that will not inhibit PCR reactions. Here, we would provide method to indicate PCR inhibition and quality of DNA samples. Defined copy number of synthesized DNA were serially dispensed into 96 wells PCR plates by using bioprinting technology. Potential PCR inhibitor substances and DNA samples were added in the prepared PCR plates containing synthesized DNA. Positive amplifications of the synthesized DNA would indicate the absence or lack of potential inhibitory effects on PCR reactions. Our results showed several amplification failures showing inhibitory effects of chemical substances at certain concentrations. This method has enabled us to examine the effects of inhibitor substances remained in DNA samples on the PCR reactions. In our presentation, several types of potential PCR inhibitor substances including its inhibitory effects would be compared and discussed. Presenter: Michie Hashimoto, Ricoh Co., Ltd, Kawasaki, Japan, Email: michie.mm.hashimoto@jp.ricoh.com P-T-082 Steven Gu , Robin Sun , Jeanne Li , Herbalife Health Products Ltd, Suzhou, China; Peter Chang , Gary Swanson , Herbalife Nutrition, Torrance, CA, USA A Single Laboratory Validation of a Test Method for Determination of Sodium Hyaluronate in Functional Food Sodium hyaluronate is a physiological glycosaminoglycan used for lubrication in articular cavity. It is widely used in different

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