AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

P-T-084 Michelle Rosauer , Burku Yordem , Hannah Bakken , Lisa Monteroso , 3M, St Paul, MN, USA Evaluation of the 3M™ Clean-Trace™ Hygiene Monitoring and Management System on Three Surfaces ATP bioluminescence is a quick and objective system of cleaning verification in food and beverage manufacturing. Continuous monitoring and data-based decisions are mandated in legisla- tions such as U.S. Food and Drug Administration Food Safety Modernization Act following the risk-based approach and in Europe by EC 852/2004. Verification of sanitary protocols through ATP bioluminescence is a rapid way to meet regu- latory requirements and customer expectations. To evaluate performance of the 3M™ Clean-Trace™ Hygiene Monitoring and Management System on multiple surfaces. Studies for pure analyte LOD, microbial response and matrix response from three surfaces, selectivity, product consistency and stability, instru- ment variation and robustness, and an inhibition study were conducted. Data collected indicate that the 3M™ Clean-Trace™ Hygiene Monitoring and Management System can detect ATP at various concentrations in ground beef, sliced deli turkey, orange juice, plain yogurt and apple turnover on plastic, ceramic and stainless-steel surfaces, and from microbial source,s on ceramic and stainless-steel surfaces. The LOD of the method was deter- mined to be 3.03 fmole ATP/swab. However, a statistically significant difference is seen between 0 fmole and 1 fmole data sets using ANOVA ( p = 0.000 ). Results as seen on three surfaces and with other testing supported the system’s claimed limit of detection and intended use. Presenter: Michelle Rosauer, 3M, St Paul, MN, USA, Email: mlrosauer@mmm.com PERFORMANCE TESTED METHODS SM P-T-085 Jessica Williams , Ana-Maria Leonte , Thermo Fisher Scientific, Basingstoke, United Kingdom; Ben Bastin , Q Laboratories, Inc., Cincinnati, OH, USA; Maryse Rannou , Muriel Bernard , ADRIA, Quimper, France AOAC-RI PTM and NF Validation of the Thermo Scientific™ SureTect™ Salmonella Species PCR Assay Using the QuantStudio™ 5 PCR Instrument The Thermo Scientific™ SureTect™ Salmonella species PCR Assay enables the detection of Salmonella spp. from a variety of food and environmental samples. Extension studies were peformed to extend the AOAC-RI PTM and NF Validation claims to include the use of the new Applied Biosystems™ QuantStudio™ 5 Real-Time PCR Food Safety System. The validation studies were conducted per the AOAC-RI PTM, NF Validation and ISO 16140-2:2016 guidelines. For the SureTect™ Salmonella species method, all samples underwent an enrichment step followed by direct lysis. Following direct lysis, PCR was run and results were automatically interpreted by the software. The reference method was conducted per EN ISO 6579-1:2017. A total of 180 (AOAC-RI PTM) and 456 (NF Validation) food and environmental samples were tested using the SureTect™ Salmonella species method and EN ISO 6579-1:2017. The SureTect™ Salmonella species method

pharmaceutical and medical fields attribute to its properties of cell motility and wound healing. Recently, sodium hyaluronate is also used in cosmetic and dietary supplement because of its moisturizing, regenerative and protective effects. Methods reported for sodium hyluronate or hyaluronaic acid (HA) determination include Size Exclusion Liquid Chromatography (SEC) with UV detection, hydrolyzing with hyaluronidase for UV detection, and HPLC with UV or FL detection. In food industry, the complex matrix is a challenge for measuring it in dietary supplement, hence, an easy, accurate and specified quan- titative method for determination of sodium hyaluronate is a critical need for food-labeling and surveillance. Since microbial hyaluronidases (hyaluronate lyase, EC 4.2.2.1) degrade sodium hyaluronate or HA only to disaccharide, a HPLC with Refractive Index Detector (RI) was developed to determine sodium hyaluro- nate by quantifying the content of disaccharide using a column of strong cation-exchange resin. A Single laboratory validation (SLV) was performed on Beauty Powder formula containing sodium hyaluronate to evaluate method specificity, precision, accuracy, linearity and ruggedness. This test method showed a linearity range from 8 µg/mL to 80 µg/mL, the average recov- ery for sodium hyaluronate is 97% in general. Presenter: Peter Chang, Herbalife Nutrition, Torrance, CA, USA, Email: peterch@herbalife.com P-T-083 Steven Gu , Robin Sun , Jeanne Li , Herbalife Health Products Ltd, Suzhou, China; Peter Chang , Gary Swanson , Herbalife Nutrition, Torrance, CA, USA A Single Laboratory Validation of a Test Method for Determination of Cyanocobalamin (Vitamin B12) in Protein Solid Drink by LC/MS Vitamin B12 in dietary supplement is usually kept at a very low concentration, such as ppb level per severing in protein solid drink. Typically, HPLC with UV-Vis detector is used for testing vitamin B12 in dietary supplement. LC/MS and microbiologi- cal method are used for testing at lower concentration. Given the high sensitivity and high-throughput, the LC/MS shows the advantage for vitamin B12 test, however, due to matrix issue, the interference and efficient extraction contribute to the most challenge for LC/MS technology. In this study, an easy prepared acetate buffer solution was used to extract vitamin B12, the buffer is not only to protect B12 from degradation, but to help precipitate protein to minimize interference, with aid of pectase, some ingredients like polysaccharide was degraded to lower viscosity of sample preparation, that make the ready to easily pass through solid phase extraction (SPE) to concentrate vitamin B12. A different product ion at 147.1 was utilized to quantify vitamin B12. A single laboratory validation (SLV) was performed on Protein Drink Powder (Mango Flavor) formula to evaluate method specificity, precision, accuracy, linearity and ruggedness This test method demonstrated a linearity range from 1 to 10 ppb, the average recovery is 95.6%. Presenter: Peter Chang, Herbalife Nutrition, Torrance, CA, USA, Email: peterch@herbalife.com

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