AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

demonstrated equivalent performance to the EN ISO 6579- 1:2017 reference method during the AOAC-RI PTM and NF Validation by AFNOR certification studies. The alternative method proved to be a suitable substitute to the EN ISO 6579- 1:2017 reference method for Salmonella species detection. Presenter: Jessica Williams, Thermo Fisher Scientific, Basingstoke, United Kingdom, Email: jessica.williams@thermofisher.com P-T-086 Jessica Williams , Ana-Maria Leonte , Thermo Fisher Scientific, Basingstoke, United Kingdom; Ben Bastin , Q Laboratories, Inc., Cincinnati,, OH, USA; Maryse Rannou , Muriel Bernard , ADRIA, Quimper, France AOAC-RI PTM and NF Validation of the Thermo Scientific™ Listeria Monocytogenes PCR Assay Using the QuantStudio™ 5 PCR Instrument The Thermo Scientific™ SureTect™ Listeria Monocytogenes PCR Assay enables the detection of Listeria Monocytogenes from a variety of food and environmental samples. Extension studies were performed to validate the use of the SureTect™ Listeria Monocytogenes method with the new Applied Biosystems™ QuantStudio™ 5 Real-Time PCR Food Safety System. The validation studies were conducted per the AOAC-RI PTM, NF Validation and ISO 16140-2:2016 guidelines. For the alternative method, all samples underwent an enrichment step followed by direct lysis. Following direct lysis, PCR was run and results were automatically interpreted by the software. The reference method was conducted according to EN ISO 11290-1:2017. A total of 150 (AOAC-RI PTM) and 387 (NF Validation) food and environmental samples were tested using the SureTect™ Listeria Monocytogenes and EN ISO 11290-1:2017 methods. The alternative method demonstrated equivalent performance for all human food and environment samples analysed in compar- ison to the EN ISO 11290-1:2017 reference method, during the AOAC-RI PTM and NF Validation by AFNOR certification studies. The SureTect™ Listeria Monocytogenes method proved to be a suitable substitute to the EN ISO 11290-1:2017 reference method for L. monocytogenes detection. Presenter: Jessica Williams, Thermo Fisher Scientific, Basingstoke, United Kingdom, Email: jessica.williams@thermofisher.com P-T-087 Jessica Williams , Ana-Maria Leonte , Thermo Fisher Scientific, Basingstoke, United Kingdom; Ben Bastin , Q Laboratories, Inc., Cincinnati, OH, USA; Maryse Rannou , Muriel Bernard , ADRIA, Quimper, France AOAC-RI PTM and NF Validation of the Thermo Scientific™ Listeria Species PCR Assay Using the QuantStudio™ 5 PCR Instrument The Thermo Scientific™ Listeria species PCR Assay enables the detection of Listeria spp. from a broad range of food and envi- ronmental samples. Extension studies have been performed to extend the AOAC-RI PTM and NF Validation claims to include the use of Applied Biosystems™ QuantStudio™ 5 Real-Time PCR Food Safety System. The validation studies were conducted per the AOAC-RI PTM, NF Validation and ISO 16140-2:2016 guidelines. For the SureTect™ Listeria species method, all samples underwent an enrichment step followed by direct lysis. Following

direct lysis, PCR was run and results were automatically inter- preted by the software. The reference method was conducted according to EN ISO 11290-1:2017. A total of 150 (AOAC-RI PTM) and 378 (NF Validation) food and environmental samples were tested using the SureTect™ Listeria species method and EN ISO 11290-1:2017 method. The SureTect™ Listeria species method demonstrated equivalent performance in compari- son to the EN ISO 11290-1:2017 reference method during the AOAC-RI PTM and NF Validation by AFNOR certification studies. The SureTect™ Listeria species method proved to be a suitable substitute to the EN ISO 11290-1:2017 reference method for Listeria species detection. Presenter: Jessica Williams, Thermo Fisher Scientific, Basingstoke, United Kingdom, Email: jessica.williams@thermofisher.com P-T-088 Jessica Williams , Ana-Maria Leonte , Thermo Fisher Scientific, Basingstoke, United Kingdom; Ben Bastin , Q Laboratories, Inc., Cincinnati, OH, USA AOAC-RI PTM Validation of the Thermo Scientific™ SureTect™ E. coli O157:H7 PCR Assay Using the Applied Biosystems™ QuantStudio™ 5 PCR Instrument The Thermo Scientific™ SureTect™ E. coli O157:H7 PCR Assay has completed an AOAC PTM method modification study for the detection of Escherichia coli O157:H7 from a variety of foods using the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR Food Safety System. The validation extension study included an inclusivity and exclusivity evaluation and an unpaired method comparison using representative matrices of raw ground beef (375 g) and fresh spinach (25 g). The candidate method was compared to the ISO 16654:2001 for raw ground beef and USDA/FSIS MLG 5.09 for spinach. Probability of Detection statistical analysis at 95% confidence levels demonstrated no statistically significant differences between the alterna- tive and reference methods during the method comparison study. Inclusivity tested demonstrated all 52 E. coli O157:H7 isolates were correctly identified and all 30 exclusivity isolates were correctly excluded. The candidate method using the QuantStudio™ 5 PCR Instrument for PCR and RapidFinder™ anal- ysis software for data analysis proved to be a suitable substitute to the reference methods for E. coli O157:H7 detection in raw ground beef, raw beef trim, fresh spinach, and apple juice. Presenter: Jessica Williams, Thermo Fisher Scientific, Basingstoke, United Kingdom, Email: jessica.williams@thermofisher.com P-T-089 Jessica Williams , Ana-Maria Leonte , Thermo Fisher Scientific, Basingstoke, United Kingdom; Maryse Rannou , Muriel Bernard , ADRIA, Quimper, France NF Validation of the Thermo Scientific™ SureTect™ Cronobacter Species PCR Assay Using the Applied Biosystems™ QuantStudio™ 5 PCR Instrument NF Validation by AFNOR certification has validated the Thermo Scientific™ SureTect™ Cronobacter species PCR Assay for the detection of Cronobacter species in powdered infant formula samples (PIF) (10g and 300g) and production environmen- tal samples using the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR Food Safety System in accordance with ISO

82 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL