AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

16140:2016. Matrices of 10 g PIF with and without probiotics, 300 g PIF with and without probiotics and production environ- ment samples were tested during this extension study. A total of 208 milk powder and environmental samples were tested using the alternative and ISO 22964:2017 methods. Fourteen positive deviations were observed and confirmed with culture methods. The sensitivity of the SureTect™ Cronobacter method was demon- strated to be 91.7% with a relative trueness of 89.4% and a false positive ratio of 6.3%. The SureTect™ Cronobacter method demonstrated equivalent performance for all samples analysed in comparison to the ISO 22964:2017 reference method, during NF Validation by AFNOR certification studies. The SureTect™ Cronobacter method proved to be a suitable substitute to the ISO 22964:2017 reference method for Cronobacter species detection. Presenter: Jessica Williams, Thermo Fisher Scientific, Basingstoke, United Kingdom, Email: jessica.williams@thermofisher.com P-T-090 Jessica Williams , Ana-Maria Leonte , Thermo Fisher Scientific, Basingstoke, United Kingdom; François Le Nestour , Microsept Laboratory, Le Lion d’Angers, France Thermo Scientific™ Listeria Precis™ Enumeration Method NF Validation in Accordance with EN ISO 16140-2:2016 NF Validation by AFNOR certification has previously vali- dated the Thermo Scientific™ Listeria Precis™ Enumeration method (alternative method) for the enumeration of Listeria Monocytogenes from a broad range of foods and environmen- tal samples. To assess performance in comparison to the ISO 11290-2:2017 reference method, and renew the validation in line with EN ISO 16140-2:2016. The relative trueness study (74 samples) results satisfied the requirements of EN ISO 16140- 2:2016. The accuracy profile tolerance intervals were within the EN ISO 16140-2:2016 acceptability limits for all six matrix/ strain combinations. Inclusivity testing (50 L. monocytogenes ) isolates were all successfully detected and exclusivity testing (31 non-target isolates) showed no cross reactions. The ILS results from 10 laboratories were scatter plotted using reference method vs the alternative method; the data meets the Acceptability Limits (AL) for all levels of contamination, therefore is equivalent to the reference method. The Listeria Precis™ Enumeration method is equivalent to the EN ISO 11290-2:2017 reference method for the enumeration of Listeria Monocytogenes from a broad range of foods and environmental samples. Presenter: Jessica Williams, Thermo Fisher Scientific, Basingstoke, United Kingdom, Email: jessica.williams@thermofisher.com P-T-091 Kazunori Nishimoto , Natsumi Tanaka , Wataru Saito , Mikio Bakke , Kikkoman Biochemifa Company, Noda-shi, Chiba, Japan Validation Study of LuciPac A3 Surface for Hygiene Monitoring Through Detection of ATP, ADP, and AMP from Stainless Steel Surface: AOAC Performance Tested Method SM 051901 ATP is frequently used for hygiene monitoring tests in the food and medical industries. However, ATP was hydrolyzed to ADP, AMP in some samples, and the low ATP of the samples

resulted in false negative finding on the ATP test. It is impera- tive to develop a hygiene indicator that does not cause false negative results. Total adenylate (ATP+ADP+AMP) could be a more reliable indicator. Kikkoman Biochemifa Company has developed a next generation ATP hygiene monitoring kit for detecting total adenylate, and designated it LuciPac A3 Surface. This system was validated for Performance Tested Methods SM certification. The LuciPac A3 Surface System was evaluated for the limit of detection (LOD) for each adenylate and the detection of food or microbial residues on stainless steel surfaces. Pure analyte studies performed by the method developer and the independent laboratory showed good linearity (R 2 > 0.9862) and repeatability (RSD r < 20% for ≥2.5 fmol/assay). The LOD values for each adenylate were around 10 Relative Light Units or 2.5 fmol/assay. The repeatability in the method developer laboratory for the matrix study (raw chicken breast, sliced deli ham, orange juice, yogurt and apple pie) and the microbial study ( Cronobacter sakazakii , Lactobacillus acidophilus and Saccharomyces cerevisiae ) were 8-30 and 10-35%, respec- tively. Interference by disinfectants, influence of adenylate analogues, instrument variation, lot-to-lot consistency and accel- erated stability were also confirmed. Presenter: Kazunori Nishimoto, Kikkoman Biochemifa Company, Noda-shi, Chiba, Japan, Email: knishimoto@mail.kikkoman.co.jp P-T-092 Sabina Pederiva , Paola Brizio , Stefania Squadrone , Anna Riva , Stefania Gavinelli , Marco Rizzi , Angelo Ferrari , Maria Cesarina Abete , Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, CReAA, National Reference Centre for the Surveillance and Monitoring of Animal Feed, Turin, Italy Method Validation for the Determination of Total Mercury in Food and Feed by Direct Mercury Analysis (DMA) According to UNI CEI EN ISO/IEC 17025:2018 and Regulation (EU) 2018/73 Mercury (Hg) is a toxic element, of both natural and anthro- pogenic origin, which can bio accumulate along the food chain. In 2017 the European Commission issued the DG SANTE/11813/2017 concerning the quality control for pesti- cide residues and analysis in food and feed and in 2018 the Regulation (EU) 2018/73 updated maximum residue levels for mercury compounds. The aim of this work is the development and validation of a method of analysis of Hg in different matrices according to UNI CEI EN ISO/IEC 17025:2018. As provided by DG SANTE a representative sample for each commodity was selected to develop the method. After the homogenization 0.0500-0.2000 g of sample were weighted in duplicated and analysed via atomic absorption spectrometry by Direct Mercury Analyzer (DMA-80), at 253.65 nm. During each analytical session standard solutions and blank reagents were processed to verify the performances of the methods and certified reference materials were analysed to define the field of measurement. The following parameters were evaluated: specificity, LOQ and LOD, recovery, repeatability and uncertainty. The validated method fulfills national and international requirements. Mercury can be detected in the range of 0.010–5.0 mg/kg with a LOQ proper for monitoring trace level. A wide range of food matrices identi- fied by the DG SANTE/11813/2017 were tested to validate the

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