AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Tuesday

technique, allowing the development of a robust method to be used in the frame of Hg monitoring programs. Presenter: Sabina Pederiva, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, CReAA, National Reference Centre for the Surveillance and Monitoring of Animal Feed, Turin, Italy, Email: sabina.pederiva@izsto.it P-T-093 Katherine Stenerson , Olga Shimelis , Teresa Marsala , MilliporeSigma, Bellefonte, PA, USA Measuring Ethanol in Kombucha Tea by Headspace Solid Phase Microextraction (HS-SPME): A Walk Through Method Development and Validation Kombucha tea has been gaining in popularity throughout the United States and Europe. The fermentation process used in its production results in a tangy, sometimes fizzy drink that is rich in probiotics. Yeast present in the Kombucha consumes sugars which in turn produces alcohol. In the United States, if the alcohol level is > 0.5%, it is considered an alcoholic bever- age and is thus subject to appropriate laws and regulations. Kombucha manufacturers are held responsible for the ethanol level in their products and are encouraged to have testing done using a reliable method. Since Kombucha can contain a vari- ety of flavorings and additives, the method must be accurate with the presence of a variety of matrix components. Recently, AOAC adopted as first action a headspace solid phase microex- traction (HS-SPME) method for analysis of ethanol in Kombucha. This method was developed and validated using a variety of Kombucha tea products. Analysis was done by GC-MS which allowed use of isotopically labeled ethanol as an internal stan- dard, thus ensuring a high level of accuracy. The data presented will summarize the steps done to develop the SPME method, as well as the results of the single-laboratory validation study. In addition, the method was also adapted to the testing of wine samples. Using the appropriate dilution, it was found to be appli- cable to this matrix as well. Presenter: Katherine Stenerson, MilliporeSigma, Bellefonte, PA, USA, Email: katherine.stenerson@milliporesigma.com P-T-094 R. Lucas Gray , Chelsea Kronemeyer , Alex Kostin , Gabrielle Anderson , Brooke Roman , Frank Klein , Robert Donofrio , Neogen Corp., Lansing, MI, USA Validation of the Reveal ® Q+ for DON Method for Quantitative Determination of Deoxynivalenol in Grains and Grain Products A Performance Tested Method SM validation was conducted on Neogen’s Reveal ® Q+ for DON quantitative immunochromato- graphic test for determining deoxynivalenol contamination in grains. The results showed the test to be accurate and consistent,

and the test has been accepted for PTM status and assigned PTM number 071901. The test’s performance was validated on naturally contaminated corn and wheat samples. Across a reference level range of 0.5 to 34.5 ppm, mean recovery ranged from 90 to 104%. The limit of detection was calculated as 0.014 ppm in wheat and 0.037 ppm in corn, and the limit of quantitation was calculated as 0.042 ppm in corn and 0.11 ppm in wheat. Both commodities had a linearity R 2 value of 0.999. Spiked samples of eight additional grains were analyzed from 0.5 to 30 ppm, with mean recovery ranging from 90 to 109%. Cross-reactivity tests showed no detection of or interference by other mycotoxins. All primary validation results were supported by independent laboratory testing, and consistency and stability studies showed consistent lot-to-lot performance across the test’s 18-month expiry period. Presenter: R. Lucas Gray, Neogen Corp., Lansing, MI, USA, Email: lgray@neogen.com P-T-095 Andriy Tkachenko , Jake Guag , Renate Reimschuessel , U.S. Food and Drug Administration, Laurel, MD, USA Blinded Method Test (BMT) is an Additional Tool in Method Validation Process Food and Drug Administration’s (FDA) Veterinary Laboratory Investigation and Response Network (Vet-LIRN) comprises 43 veterinary diagnostic laboratories across North America. Laboratories assist with investigations into potential food/feed contamination events and mainly focus on analysis of animal diagnostic samples. To evaluate performance of new chemis- try methods, Vet-LIRN Program Office (VPO) conducts Blinded Method Tests (BMTs) with the method originating laboratory followed by a multi-laboratory BMT. In both Single- and Multi-laboratory BMTs test samples are prepared by VPO and analyzed by participants in a blinded manner. Single-laboratory BMTs have been identified as an essential prerequisite exercise prior to multi-laboratory exercises because they ensure the method works well and may bring to light important aspects to include when transferring the method to other laboratories. The VPO can, within a relatively short time period, evaluate major characteristics of method performance with a high degree of confidence. BMTs were successfully applied for both quanti- tative and qualitative methods based on different instrument platforms confirming the great flexibility and adaptability of the BMT approach. Key BMT features include: preparation of the test samples, capturing and processing data, and application of project management tools. Presenter: Andriy Tkachenko, U.S. Food and Drug Administration, Laurel, MD, USA, Email: andriy.tkachenko@fda.hhs.gov

84 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL