AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

guiding significance for the development of fish falvor and fragrance and improvement of flavor quality of fish meat. In this paper, the Shimadzu GCMS-TQ8050 triple quadrupole GC/MS and AOC-6000 multi-function autosampler were combined with the off-flavor database to determine the odor components in three kinds of fish meat. Presenter: Eberhardt Kuhn, Shimadzu USA, Columbia, MD, USA, Email: erkuhn@shimadzu.com P-W-017 Natsuyo Asano , Eishi Imoto , Mami Okamoto , Shimadzu Japan, Kyoto, Japan; Mikie Shima , AiSTI Science, Wakayama, Japan; Jun Watanabe , Shimadzu USA, Wakayama, Japan; Alan Owens , Shimadzu USA, Columbia, MD, USA Fast and Simlutaneous LC-MS/MS Analysis for Veterinary Drugs in Meat Combined with STQ Method Veterinary drugs are used for therapeutic and growth promo- tion purposes for animals or fish. To provide assurance that food from animals is safe in regard to veterinary medicine residues, regulatory authorities have established Maximum Residue Limits (MRL) for certain drugs in target tissues and animal species. Veterinary drugs analysis commonly uses liquid chromatography coupled to mass spectrometer which is fast, highly sensitive and highly selective. This work describes the application of high-throughput LC-MS/MS system utilizing fast polarity switching. Faster, easier and high precision total workflow was investigated with QuEChERS method combined with solid-phase extraction cartridge to enhance purification efficiency. Presenter: Alan Owens, Shimadzu USA, Columbia, MD, USA, Email: amowens@shimadzu.com P-W-019-A Tasuku Murata , Koretsugu Ogata , Shimadzu Japan, Kyoto, Japan; Yuji Nagashima , Niigata Agro-Food University, Kyoto, Japan; Alan Owens , Shimadzu USA, Columbia, MD, USA Rapid and Easy Analysis of Tetrodotoxin by Direct Probe Ionization/Tandem Mass Spectrometry (DPiMS) Tetrodotoxin (TTX), pufferfish toxin, is one of the potent natural toxins, often causing food poisoning by ingesting. In analysis of TTX, LC-MS/MS is mainly used with high precision and sensitiv- ity. However, it needs cumbersome sample preparation including extraction, cleaning-up and so on. The preferable way for TTX analysis is “no sample preparation” without loss of toxin. Ambient ionization techniques have been recently improved and probe electrospray ionization (PESI) require no sample prepa- ration of tissue samples. PESI was developed by Hiraoka et al. in 2007, which uses an ultra-thin needle probe (tip diameter is under 700 nm) as a sampling and ionization units. Therefore, PESI is possible to achieve “no sample preparation” analysis. For applications using PESI, especially combinational use of PESI with highly selective mass spectrometers such as tandem mass spectrometry and high-resolution mass spectrometry are mainly introduced. In this poster, we introduce the principle of PESI and its application for food safety, the ultra-rapid and easy analysis of tetrodotoxin for tissue samples of pufferfish without

tedious sample preparation. The ultra-rapid and easy analysis of tetrodotoxin was achieved for tissue samples of pufferfish without complicated sample preparation by direct probe ionization-tan- dem mass spectrometry. Presenter: Alan Owens, Shimadzu USA, Columbia, MD, USA, Email: amowens@shimadzu.com P-W-019-B Anna Plocicka-Okladlo , Katarzyna Banaszewski , NOW Foods, Bloomingdale, IL, USA Arsenic Speciation in Krill and Other Marine Oils by Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry The content of total and inorganic arsenic was determined in krill oil and the newly developed speciation method was carried out through a single laboratory validation. Krill oil samples were prepared by microwave digestion with the use of diluted nitric acid and hydrogen peroxide. Chromatographic separation of five arsenic species: arsenobetaine (AsB), dimethylarsinic acid (DMA), arsenite (AsIII), monomethylarsonic acid (MMA), and arsenate (AsV) was achieved by high-performance liquid chromatography (HPLC) using an anion exchange column and strongly basic carbonate eluent. Detection of the arsenic species was performed by inductively-coupled plasma mass spectrome- try (ICP-MS). Signal drift was corrected by post column addition of an internal standard. Method performance was evaluated using % mass balance of speciated arsenic versus total arsenic, and recovery of a fortified analytical portion (FAP). The average % mass balance from replicate preparations of krill oil samples was 95.5% and the recovery of FAP of all five species met the acceptance criteria of 95-105%. This procedure should prove useful when quantitating inorganic arsenic species in marine oils. Presenter: Anna Plocicka-Okladlo, NOW Foods, Bloomingdale, IL, USA, Email: anna.okladlo@nowfoods.com

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