AOAC 133rd Annual Meeting - Final Program

Poster Abstracts | Wednesday

or pass-through SPE) may be suitable for accurate determina- tion of hundreds of analytes from complex matrices. Although simple, such extraction and cleanup methods are not fool-proof; variables in the extracts, such as water content, protein content, pigment content, and lipid content, need to be considered to maximize cleanup and avoid recovery losses of individual compounds or compound classes. In this presentation, multi-resi- due veterinary drug analysis will be discussed with emphasis on the optimization of a pass-through SPE cleanup protocol. Presenter: Michael Young, Waters Corp., Milford, MA, USA, Email: michael_s_young@waters.com P-W-033 Wendy Yuan , Mérieux NutriSciences Corp., Burnaby, BC, Canada Determination and Confirmation of Antibiotic and Antiviral Drug Residues in Chicken Jerky Pet Treat A LC-MS/MS method for the analysis of three antiviral drug residues (amantadine, rimantadine, memantine) and five antibi- otic drug residues (enrofloxacin, sulfaclozine, sulfaquinoxaline, tilmicosin, trimethoprim) in chicken jerky pet treat was devel- oped. Deionized water is added into homogenized chicken jerky pet treat sample, and mixed well. The antibiotic and antiviral drug residues were extracted using acidic acetonitrile, and then purified using dispersive column. The analytes were analyzed with an AB Sciex API 6500 plus MS/MS coupled with an Agilent 1260 Infinity LC system. The average recoveries for amantadine (internal standard amantadine-d15 is used) in forti- fied samples ranged from 85 to 101%, and the RSD was ≤ 4.6%. The quantification limit of this method for all analytes is 1 µg/kg, and the analysis range is 1 to 200 µg/kg. Presenter: Wendy Yuan, Mérieux NutriSciences Corp., Burnaby, BC, Canada, Email: wendy.yuan@mxns.com P-W-034 Ronald Niemeijer , Thomas Nick , Thomas Hektor , R-Biopharm AG, Darmstadt, Germany No Big (Fattening) Surprise: The Hormone Zilpaterol is Prohibited in the E The β -agonist Zilpaterol as active ingredient of Zilmax is used to increase the bodyweight gain of cattle in the last feeding period. Three days before slaughtering, the drug should be withdrawn. It is worth pointing out that Zilpaterol should not be used for dairy cattle. Zilpaterol is used at a dosage of around 7.5 mg/ kg in several countries around the world, while it is strictly forbidden in the EU. The reason is the potential risk of cardio- vascular effects after Zilpaterol intake. The only way to ensure safe food is an efficient screening of a large amount of samples around the world. A cost efficient and reliable testing method for this purpose is our new competitive enzyme immunoassay RIDASCREEN ® Zilpaterol. This assay allows the quantitative detection of Zilpaterol in milk, urine and blood to observe the drug concentration in the last feeding period. The ELISA is furthermore well suited for the import control of meat. The test has the following limit of detections: meat (bovine, porcine) ca. 150 ng/kg; urine (bovine) ca. 550 ng/L; serum/plasma (bovine)

ca. 240 ng/L. The corresponding recoveries are: meat (bovine, porcine) ca. 103%; urine (bovine) ca. 90%; serum/plasma ca. 107%. With these properties, the test is in accordance with the limits advised by the Community Reference Laboratories. Thus, the RIDASCREEN ® Zilpaterol helps to keep the level of harmful residues low in our food. Presenter: Ronald Niemeijer, R-Biopharm AG, Darmstadt, Germany, Email: r.niemeijer@r-biopharm.de P-W-035 Clare Ho , Chi-Kin Koo , Tony Chong-Ho Lam , Government Laboratory HKSAR, Hong Kong, Hong Kong Pesticide Multi-Residue Analysis by LC-MS/MS and LC-HR-MS with Multiple Calibration Techniques Quality control criteria are evolved subsequent to the imple- mentation of analytical quality criteria (AQC) documents such as CAC/GL-90 2017 and SANTE/11813/2017. Second analysis or re-analysis is recommended for regulatory enforce- ment data. Procedural-wise, validation of blank standard addition overlaps with procedural matrix-matched calibration; cohort validation is sporadically demonstrated in literature. Accordingly, calibration residues and matrix effect towards calibration bias are to be harmonized within +/-20%. This presentation aims to demonstrate a consolidated validation approach on pesticide residues analysis in fruits and vegetables fulfilling new data quality objectives. Presenter: Tony Chong-Ho Lam, Government Laboratory HKSAR, Hong Kong, Email: chlam2@govtlab.gov.hk P-W-036 Alan Barnes , Neil Loftus , Shimadzu Corp., Manchester, UK, Milton Keynes, United Kingdom; Steve Williams , Concept Life Sciences, Cambridge, United Kingdom; Robert Clifford , Shimadzu Scientific Instruments, Inc., Columbia, MD, USA Targeted Data Independent Acquisition for Pesticide Quantitation Using High-Resolution Accurate Mass Acquisition LC-MS/MS QTOF Analysis Pesticide monitoring programs are designed to identify and quantify pesticides within a defined regulatory framework. To minimize the risk to public health, maximum residue levels (MRL’s) have been set for pesticides in food products/groups within the EU. In this paper, a panel of over 200 pesticides were quantified using high-resolution accurate MS and MS/MS anal- ysis in agreement with SANTE/11813/2017. All pesticides in the panel and were detected at or below the reporting limit of 0.01 mg/kg for all target compounds. Presenter: Alan Barnes, Shimadzu Corp., Manchester, UK, Milton Keynes, United Kingdom, Email: Neil.Loftus@shimadzu-mso.com P-W-037 Sunil Singh , Durvesh Sawant , Prashant Hase , Sanket Chiplunkar , Nitish Suryawanshi , Jitendra Kelkar , Pratap Rasam , Shimadzu Analytical (India) Pvt. Ltd, Mumbai,

94 SEPTEMBER 6–12, 2019 SHERATON DENVER DOWNTOWN HOTEL